Matsuzawa S, Kimura H, Tu C Y, Kitamori T, Sawada T
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
J Immunol Methods. 1993 May 5;161(1):59-65. doi: 10.1016/0022-1759(93)90197-f.
Dot-immunobinding assays of IgE and CEA were performed by a conventional dot-ELISA technique with diaminobenzidine staining, and the quantitative results were compared by densitometry and a new, spectroscopic, optical beam deflection (OBD) method using the same membrane. It was possible with the OBD method to detect quantities of these substances at least ten times smaller than with densitometry. Better intra-assay reproducibility for IgE and CEA measurements was obtained by the OBD method. The measurable ranges of the OBD method was broader than that of densitometry, because dark bands caused OBD in proportion to their color densities. When the dot-immunobinding assay with OBD measurement for CEA was also compared with a microtube ELISA using biotin-avidin conjugates, the sensitivities and reproducibilities of the two methods were found to be similar, with a correlation coefficient of 0.991.
采用常规斑点酶联免疫吸附测定（dot - ELISA）技术，以二氨基联苯胺染色进行IgE和癌胚抗原（CEA）的斑点免疫结合测定，并通过光密度测定法以及使用同一膜的新的光谱光束偏转（OBD）方法比较定量结果。采用OBD方法能够检测到这些物质的量至少比光密度测定法小十倍。通过OBD方法获得了更好的IgE和CEA测量的批内重复性。OBD方法的可测量范围比光密度测定法更宽，因为深色条带引起的光束偏转与其颜色密度成比例。当将采用OBD测量的CEA斑点免疫结合测定与使用生物素 - 抗生物素蛋白缀合物的微量管酶联免疫吸附测定进行比较时，发现两种方法的灵敏度和重复性相似，相关系数为0.991。
