Zhou P, Deng Y, Zeng Y
Department of Chemistry, Wuhan University, China.
Fresenius J Anal Chem. 2000 Jul;367(5):422-5. doi: 10.1007/s002160000362.
Dot-immunobinding assay of hepatitis B surface antigen (HBsAg) was performed by a conventionally dot-ELISA technique with 4-chloro-1-naphthol staining, and the quantitative results were measured by an indirect photoacoustic method. In this method, a 2-mW helium-neon laser was applied to provide the excitation beam, the blue spots on the membrane were detected in a piezoelectric transducer-based photoacoustic cell. The operational conditions of measurement were optimized. A significant difference from the negative human serum was obtained for 50 pg in HBsAg detection. The method proposed provides a sensitive quantitative technique for dot-immunobinding assay.
采用常规斑点酶联免疫吸附测定（dot-ELISA）技术，以4-氯-1-萘酚染色法进行乙型肝炎表面抗原（HBsAg）的斑点免疫结合测定，并通过间接光声法测定定量结果。在此方法中，使用2毫瓦的氦氖激光提供激发光束，在基于压电换能器的光声池中检测膜上的蓝色斑点。优化了测量的操作条件。在HBsAg检测中，50皮克时与阴性人血清有显著差异。所提出的方法为斑点免疫结合测定提供了一种灵敏的定量技术。
