一种用于斑点免疫结合测定（斑点酶联免疫吸附测定）定量的简易反射式显微光度计。
A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.
作者信息
Ferreira C S, Ferreira M U
机构信息
Departamento de Parasitologia, Universidade de São Paulo, Brasil.
出版信息
Braz J Med Biol Res. 1991;24(5):495-8.
PMID:1823264
Abstract
The use of a simple reflectance microphotometer adapted to quantify enzymatic dot immunobinding assays is described. The instrument is versatile and precise by clinical laboratory standards (i.e., 1.1% coefficient of variation for ten consecutive readings of a grey cardboard). The instrument provides a low-cost alternative to commercially available equipment for quantitation of dot-ELISA assays.
摘要
本文描述了一种适用于定量酶联斑点免疫结合测定的简易反射式显微光度计的使用方法。按照临床实验室标准，该仪器通用性强且精度高（即对灰色硬纸板连续进行十次读数的变异系数为1.1%）。该仪器为定量斑点酶联免疫吸附测定提供了一种低成本的市售设备替代方案。
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