Borel Y, Golan D T, Kilham L, Borel H
J Immunol. 1976 Mar;116(3):854-8.
Groups of BALB/c mice were treated with various conjugates of 2,4 dinitrophenyl (DNP) and BALB/c myeloma proteins belonging to the four subclasses of IgG (IgG1, IgG2, IgG2b, IgG3). Immediately therafter, they were challenged with DNP-keyhole limpet hemocyanin in complete Freund's adjuvant and antibody to the hapten was measured by direct and indirect hemolytic plaque assay. The results show that all subclasses of IgG are effective as tolerance-inducing carriers. However, the ability of induce tolerance is dependent upon the concentration of hapten bound to each myeloma protein. Tolerogenic conjugates suppress both direct and indirect plaque-forming cells in all types of antibodies measured (IgG1, IgG2a, IgG2b, IgGa), whereas, non-tolerogenic conjugate failed to suppress them. The intact molecule of IgG but not its fragments (Fab, F(ab)2', Fc) appear necessary as tolerance-inducing carriers. It is suggested that the ability to induce tolerance is related to the capacity of the tolerogenic conjugates to cause receptor blockade.
将BALB/c小鼠分组，用2,4-二硝基苯基（DNP）与属于IgG四个亚类（IgG1、IgG2、IgG2b、IgG3）的BALB/c骨髓瘤蛋白的各种缀合物进行处理。此后立即用完全弗氏佐剂中的DNP-钥孔戚血蓝蛋白对它们进行攻击，并通过直接和间接溶血空斑试验测量对半抗原的抗体。结果表明，IgG的所有亚类作为诱导耐受性的载体都是有效的。然而，诱导耐受性的能力取决于与每种骨髓瘤蛋白结合的半抗原的浓度。致耐受性缀合物在所有测量的抗体类型（IgG1、IgG2a、IgG2b、IgGa）中均抑制直接和间接空斑形成细胞，而无耐受性的缀合物则不能抑制它们。IgG的完整分子而非其片段（Fab、F(ab)2'、Fc）似乎是诱导耐受性的必要载体。有人提出，诱导耐受性的能力与致耐受性缀合物引起受体阻断的能力有关。
