Arizono N, Kasugai T, Yamada M, Okada M, Morimoto M, Tei H, Newlands G F, Miller H R, Kitamura Y
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
Blood. 1993 May 15;81(10):2572-8.
Ws/Ws rats have a small deletion at the tyrosine kinase domain of the c-kit gene and are deficient in both mucosal mast cells (MMC) and connective tissue-type mast cells (CTMC). The role of the c-kit receptor in the development of MMC and CTMC was investigated by infecting Ws/Ws and control +/+ rats with Nippostrongylus brasiliensis (NB), which induces T-cell-dependent mast cell proliferation. Although mast cells did not develop in the skin of Ws/Ws rats, a significant number of mast cells developed in the jejunum after NB infection. These mast cells had the MMC protease phenotype (rat mast cell protease [RMCP] I-/II+) and lacked heparin because they were not stained with berberine sulfate. Globule leukocytes were also detected in the mucosal epithelium of these rats. However, the number of MMC and the serum concentration of RMCP II in NB-infected Ws/Ws rats were only 13% and 7% of those of NB-infected +/+ rats, respectively. A small number of mast cells also developed in the lung, liver, and mesenteric lymph nodes of Ws/Ws rats after NB infection. Although mast cells in these tissues had the MMC phenotype throughout the observation period, the increased mast cells in the lung and liver of +/+ rats acquired a CTMC-like phenotype and were RMCP I+/II+, berberine sulfate+, and formalin resistant. These results indicate that the need for the stimulus through the c-kit receptor appears to be greater in the development of CTMC in the skin as well as for CTMC-like mast cells in the lung and liver than for the development of MMC.
Ws/Ws大鼠的c-kit基因酪氨酸激酶结构域存在小片段缺失，其黏膜肥大细胞（MMC）和结缔组织型肥大细胞（CTMC）均有缺陷。通过用巴西日圆线虫（NB）感染Ws/Ws大鼠和对照+/+大鼠来研究c-kit受体在MMC和CTMC发育中的作用，NB可诱导T细胞依赖性肥大细胞增殖。尽管Ws/Ws大鼠皮肤中未发育出肥大细胞，但NB感染后空肠中出现了大量肥大细胞。这些肥大细胞具有MMC蛋白酶表型（大鼠肥大细胞蛋白酶[RMCP]I-/II+）且缺乏肝素，因为它们不能被硫酸小檗碱染色。在这些大鼠的黏膜上皮中也检测到了球形白细胞。然而，NB感染的Ws/Ws大鼠中MMC的数量和RMCP II的血清浓度分别仅为NB感染的+/+大鼠的13%和7%。NB感染后，Ws/Ws大鼠的肺、肝和肠系膜淋巴结中也出现了少量肥大细胞。尽管在整个观察期内这些组织中的肥大细胞具有MMC表型，但+/+大鼠肺和肝中增多的肥大细胞获得了类似CTMC的表型，为RMCP I+/II+、硫酸小檗碱阳性且对福尔马林有抗性。这些结果表明，在皮肤中CTMC的发育以及肺和肝中类似CTMC的肥大细胞的发育过程中，通过c-kit受体的刺激需求似乎比MMC的发育更大。
