Transmitter release and calcium currents at an Aplysia buccal ganglion synapse--I. Characterization.

The Ca2+ current recorded in the presynaptic neuron (B4/B5) of an identified Aplysia synapse was characterized in terms of its activation, voltage sensitivity, Ca2+ dependence of inactivation and pharmacology. It was compared to that recorded in left upper quadrant abdominal ganglion neurons which, unlike B4/B5, display Ca2+ action potentials. The two Ca2+ currents could not be distinguished in terms of their activation threshold or voltage sensitivity. The Ca2+ current recorded in left upper quadrant neurons, however, displayed more important Ca(2+)-dependent inactivation. The peak Ca2+ current in B4/B5 neurons was significantly reduced (30-40%) by the dihydropyridine Ca2+ channel antagonist, nifedipine, while it was increased (15-20%) by the dihydropyridine Ca2+ channel agonist, BAY K8644, although none of these agents had any effect on transmitter release from B4/B5. omega-Conotoxin similarly reduced the Ca2+ current by 30-40%, but unlike nifedipine, it also caused a 50-60% reduction in B4/B5 transmitter release. The pharmacological properties of the Ca2+ current present in left upper quadrant neurons were somewhat different, as this current was unaffected by either BAY K8644 or omega-conotoxin and moderately suppressed (20%) by nifedipine.