N型和P型钙离子通道参与神经元间突触处的乙酰胆碱释放：只有N型通道是神经调质的作用靶点。
N- and P-type Ca2+ channels are involved in acetylcholine release at a neuroneuronal synapse: only the N-type channel is the target of neuromodulators.
作者信息
Fossier P, Baux G, Tauc L
机构信息
Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
出版信息
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4771-5. doi: 10.1073/pnas.91.11.4771.
Abstract
Cholinergic transmission in an identified neuro-neuronal synapse of the Aplysia buccal ganglion was depressed by application of a partially purified extract of the funnel-web-spider venom (FTx) or of its synthetic analog (sFTx). This specific blocker of voltage-dependent P-type Ca2+ channels did not interfere with the effect of the N-type Ca2+ channel blocker omega-conotoxin, which could further decrease synaptic transmission after a previous application of FTx. Similar results were obtained when the reversal order of application of these two Ca2+ channel blockers was used. Both P- and N-type Ca2+ currents trigger acetylcholine release in the presynaptic neuron. The neuromodulatory effects of FMRF-amide, histamine, and buccalin on transmitter release disappeared after the blockade of the N-type Ca2+ channels but remained still effective in the presence of FTx. These results indicate that only N-type Ca2+ channels appear to be sensitive to the neuromodulators we have identified.
摘要
应用漏斗网蜘蛛毒液（FTx）的部分纯化提取物或其合成类似物（sFTx）可抑制海兔颊神经节中一个已确定的神经-神经元突触的胆碱能传递。这种电压依赖性P型Ca2+通道的特异性阻滞剂并不干扰N型Ca2+通道阻滞剂ω-芋螺毒素的作用，在先前应用FTx后，ω-芋螺毒素可进一步降低突触传递。当使用这两种Ca2+通道阻滞剂的应用顺序颠倒时，也获得了类似的结果。P型和N型Ca2+电流均触发突触前神经元中乙酰胆碱的释放。在阻断N型Ca2+通道后，FMRF酰胺、组胺和颊肽对递质释放的神经调节作用消失，但在存在FTx的情况下仍保持有效。这些结果表明，似乎只有N型Ca2+通道对我们已鉴定出的神经调节剂敏感。
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