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用于特异性鉴定尿路病原体尿气球菌的聚合酶链反应检测方法的开发。

Development of a polymerase chain reaction test for specific identification of the urinary tract pathogen Aerococcus urinae.

作者信息

Aguirre M, Collins M D

机构信息

Department of Microbiology, Reading Laboratory, Institute of Food Research, United Kingdom.

出版信息

J Clin Microbiol. 1993 May;31(5):1350-3. doi: 10.1128/jcm.31.5.1350-1353.1993.

DOI:10.1128/jcm.31.5.1350-1353.1993
PMID:7684752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262936/
Abstract

A polymerase chain reaction test was developed for identification of the gram-positive urinary tract pathogen Aerococcus urinae. Oligonucleotide primers were based on highly specific sequences within the small-subunit rRNA gene. A confirmatory test based on hybridization of the amplified products to a highly specific internal probe was also developed.

摘要

已开发出一种聚合酶链反应检测方法,用于鉴定革兰氏阳性尿路病原体尿液气球菌。寡核苷酸引物基于小亚基rRNA基因内的高度特异性序列。还开发了一种基于将扩增产物与高度特异性内部探针杂交的确认试验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/262936/3a587766d586/jcm00017-0348-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/262936/0a28b9273ee5/jcm00017-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/262936/3a587766d586/jcm00017-0348-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/262936/0a28b9273ee5/jcm00017-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/262936/3a587766d586/jcm00017-0348-b.jpg

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本文引用的文献

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Aerococcus viridans as a causative agent of infectious endocarditis.绿色气球菌作为感染性心内膜炎的病原体。
J Hyg Epidemiol Microbiol Immunol. 1980;24(1):92-6.
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Aerococcus viridans endocarditis.绿色气球菌心内膜炎
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Acute meningitis in early childhood caused by Aerococcus viridans.幼儿期由绿色气球菌引起的急性脑膜炎。
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Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci.过氧化氢酶阴性革兰氏阳性球菌(不包括链球菌和肠球菌)的鉴定、分类及临床相关性
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Septic arthritis due to Aerococcus viridans.由绿色气球菌引起的化脓性关节炎。
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Aerococcus-like organism--an unnoticed urinary tract pathogen.类气球菌——一种未被重视的泌尿道病原体。
APMIS. 1989 Jun;97(6):539-46. doi: 10.1111/j.1699-0463.1989.tb00828.x.
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Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restriction patterns.
FEMS Microbiol Lett. 1989 Nov;53(1-2):41-5. doi: 10.1016/0378-1097(89)90363-7.
7
Phenotypic characterization, cellular fatty acid composition, and DNA relatedness of aerococci and comparison to related genera.气球菌的表型特征、细胞脂肪酸组成及DNA相关性,并与相关属进行比较。
J Clin Microbiol. 1990 Mar;28(3):416-21. doi: 10.1128/jcm.28.3.416-421.1990.
8
Aerococcus-like organism, a newly recognized potential urinary tract pathogen.类气球菌,一种新发现的潜在泌尿道病原体。
J Clin Microbiol. 1991 May;29(5):1049-53. doi: 10.1128/jcm.29.5.1049-1053.1991.
9
Phylogenetic analysis of some Aerococcus-like organisms from urinary tract infections: description of Aerococcus urinae sp. nov.对一些来自尿路感染的类气球菌属微生物的系统发育分析:尿道气球菌新种的描述
J Gen Microbiol. 1992 Feb;138(2):401-5. doi: 10.1099/00221287-138-2-401.
10
Endocarditis and osteomyelitis caused by Aerococcus viridans.绿色气球菌引起的心内膜炎和骨髓炎。
Mt Sinai J Med. 1976 May-Jun;43(3):248-52.