Grude N, Jenkins A, Tveten Y, Kristiansen B-E
A/S Telelab, Pb 1868 Gulset, 3703 Skien, University of Tromsø, Tromsø, Norway.
Clin Microbiol Infect. 2003 Sep;9(9):976-9. doi: 10.1046/j.1469-0691.2003.00704.x.
To evaluate procedures for the identification of Aerococcus urinae, we examined 24 alpha-hemolytic non-enterococcal bacterial isolates from 4373 urine samples. Published procedures were compared with 16s rRNA sequencing and biochemical profiling (BBL-Crystal-GP). 16s rRNA sequencing and BBL-Crystal-GP identified the same 13 isolates as A. urinae. Published tests failed to distinguish the 13 A. urinae isolates from eight non-A. urinae isolates; several tests exhibited no discrimination. Ciprofloxacin and trimethoprim susceptibility and growth at 45 degrees C improved discrimination. For urinary isolates, standard procedures for identification of A. urinae are redundant and insufficiently discriminatory, and may need revision. BBL-Crystal-GP is an accurate alternative.
