Pour P M, Kazakoff K, Dulaney K
Eppley Institute for Research in Cancer and Allied Disease, University of Nebraska Medical Center, Omaha 68198-6805.
Int J Pancreatol. 1993 Apr;13(2):139-42. doi: 10.1007/BF02786082.
We describe a simple immunohistochemical method for simultaneous demonstration of insulin, glucagon, somatostatin, and pancreatic polypeptide cells of the Langerhans islet in routinely fixed paraffin-embedded human and Syrian hamster pancreases. With the use of alcohol insoluble chromogens, the slides can be mounted in Permount and be kept permanently without losing the color quality and intensity. With this technique, the spatial topographical distribution and morphology of the individual pancreatic endocrine cells can be investigated in the healthy and diseased pancreas.
我们描述了一种简单的免疫组织化学方法，用于在常规固定的石蜡包埋的人及叙利亚仓鼠胰腺中同时显示胰岛的胰岛素、胰高血糖素、生长抑素和胰多肽细胞。使用醇不溶性色原，载玻片可封固于Permount中并永久保存，而不会损失颜色质量和强度。通过该技术，可以在健康和患病的胰腺中研究单个胰腺内分泌细胞的空间拓扑分布和形态。
