Dorogi P
Carl Zeiss, Inc., Microscope Div., Thornwood, NY 10594.
Am Biotechnol Lab. 1993 May;11(6):84.
At least four types of technological advances have made cell microinjection procedures precise and practical, while minimizing negative impacts on cell viability: 1) hydraulic and electromechanical (stepper) micromanipulators, which provide operators with precise spatial control; 2) high-precision syringe and automated pneumatic microinjectors, with high reproducibility in the injected volume; 3) regulated pipet-pulling devices that yield highly reproducible tip diameters, down to 0.1 microns; and 4) specially adapted microscopes in both upright and inverted configurations, which ensure high-quality observation of cellular features in brightfield and all modern contrasting techniques, while reducing movement-induced vibrations at the contact point with the cell.
至少有四种技术进步使细胞显微注射程序变得精确且实用，同时将对细胞活力的负面影响降至最低：1）液压和机电（步进）显微操作器，为操作人员提供精确的空间控制；2）高精度注射器和自动气动显微注射器，注射体积具有高重复性；3）可调节的拉针装置，可产生高度可重复的针尖直径，低至0.1微米；4）专门适配的正置和倒置配置显微镜，可确保在明场和所有现代对比技术下对细胞特征进行高质量观察，同时减少与细胞接触点处的移动引起的振动。
