Donjacour A A, Cunha G R
Department of Anatomy, University of California, San Francisco 94143-0452.
Endocrinology. 1993 Jun;132(6):2342-50. doi: 10.1210/endo.132.6.7684975.
Urogenital sinus mesenchyme (UGM) from normal, androgen receptor-positive mice appears to instructively induce prostatic morphology in urinary tract epithelium from both normal and androgen-insensitive (Tfm) mice. This indicates that epithelial androgen receptors are not necessary for prostatic development. However, the secretory function of these tissue recombinants has never been assessed. In this study antisera to androgen-dependent dorsolateral prostate (DLP) secretion were used in immunocytochemistry, Western blotting, and immunoprecipitation techniques to detect the presence of these prostatic proteins in tissue recombinants made with either normal or Tfm epithelium. In a majority of cases, UGM plus normal bladder or urethral epithelium developed into prostatic tissue that produced androgen-dependent DLP proteins. Hence UGM appeared to be capable of instructively inducing a functional prostatic phenotype in these normal heterotypic epithelia. With rare exceptions, tissue recombinants of UGM plus Tfm bladder or urethral epithelium did not produce full prostatic cytodifferentiation or mouse DLP proteins, indicating that epithelial androgen receptors are important for the final stages of morphogenesis and in the initiation of secretory function in the prostate.
来自正常的、雄激素受体阳性小鼠的泌尿生殖窦间充质（UGM）似乎能诱导正常小鼠和雄激素不敏感（Tfm）小鼠的尿路上皮形成前列腺形态。这表明上皮雄激素受体对于前列腺发育并非必需。然而，这些组织重组体的分泌功能从未被评估过。在本研究中，使用针对雄激素依赖的背外侧前列腺（DLP）分泌物的抗血清，通过免疫细胞化学、蛋白质印迹和免疫沉淀技术，来检测在由正常或Tfm上皮制成的组织重组体中这些前列腺蛋白的存在情况。在大多数情况下，UGM加上正常膀胱或尿道上皮会发育成能产生雄激素依赖的DLP蛋白的前列腺组织。因此，UGM似乎能够诱导这些正常异型上皮形成功能性的前列腺表型。除了极少数例外情况，UGM加上Tfm膀胱或尿道上皮的组织重组体并未产生完全的前列腺细胞分化或小鼠DLP蛋白，这表明上皮雄激素受体对于前列腺形态发生的最后阶段以及前列腺分泌功能的启动很重要。
