来自蜂毒的针对磷脂酶A2和蜂毒肽的鼠单克隆抗体的特性分析。
Characterization of murine monoclonal antibodies to phospholipase A2 and mellitin from bee venom.
作者信息
Müller W D, Fahlbusch B, Jung K, Gebhardt M, Jäger L
机构信息
Department of Clinical Immunology, Friedrich Schiller University Jena, FRG.
出版信息
Int Arch Allergy Immunol. 1993;101(2):153-8. doi: 10.1159/000236513.
9 murine monoclonal antibodies (MoAbs), 7 to phospholipase A2 (Pla2; Api m I) and two to mellitin (Api m III) have been produced. Specificities were demonstrated using immunoblotting and enzyme immunoassays (ELISA). Antibodies specific for Pla2 were characterized in detail. Epitope mapping of Pla2-specific MoAbs identified three independent binding regions. 5 selected MoAbs bound to one of these sites compete with specific human IgE. Two of MoAbs characterized are suitable tools to quantify Pla2 in a two-site binding assay.
已制备出9种鼠单克隆抗体（MoAbs），其中7种针对磷脂酶A2（Pla2；Api m I），2种针对蜂毒肽（Api m III）。通过免疫印迹和酶免疫测定（ELISA）证明了其特异性。对Pla2特异性抗体进行了详细表征。Pla2特异性MoAbs的表位作图确定了三个独立的结合区域。与其中一个位点结合的5种选定MoAbs与特异性人IgE竞争。所表征的两种MoAbs是在双位点结合测定中定量Pla2的合适工具。
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