Hirano T, Manabe T, Yotsumoto F, Ando K, Imanishi K, Tobe T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Hepatogastroenterology. 1993 Apr;40(2):155-8.
The redistribution of cathepsin B, a representative lysosomal enzyme, from the lysosomal pellet to the zymogen pellet in subcellular fractions and the colocalization of cathepsin B with digestive enzymes within acinar cells have been found during the early stage of caerulein-induced acute pancreatitis in the rat. This study investigated the protective effects of prostaglandins E1 and E2 on the exocrine pancreas in this experimental pancreatitis. Prostaglandin E2, but not E1, prevented the redistribution of cathepsin B along with the hyperamylasemia, and the increase in amylase and trypsinogen in the acinar cells in almost a dose-dependent manner, particularly at a dose of 100 micrograms/kg.hr of continuous infusion. These results suggest that subcellular organelle fragility is closely related to the pathogenesis of acute pancreatitis, and that prostaglandin E2 has an important cytoprotective effect on biological membranes as a stabilizer of lysosomal membrane.
在大鼠蛙皮素诱导的急性胰腺炎早期，已发现组织蛋白酶B（一种代表性的溶酶体酶）在亚细胞组分中从溶酶体沉淀重新分布到酶原沉淀，并且在腺泡细胞内组织蛋白酶B与消化酶共定位。本研究调查了前列腺素E1和E2对该实验性胰腺炎外分泌胰腺的保护作用。前列腺素E2而非E1可防止组织蛋白酶B的重新分布以及高淀粉酶血症，并几乎以剂量依赖性方式防止腺泡细胞中淀粉酶和胰蛋白酶原增加，特别是在连续输注剂量为100微克/千克·小时时。这些结果表明亚细胞器脆弱性与急性胰腺炎的发病机制密切相关，并且前列腺素E2作为溶酶体膜的稳定剂对生物膜具有重要的细胞保护作用。
