Iwato K, Kawano M M
Department of Internal Medicine, National Ohtake Hospital.
Rinsho Ketsueki. 1993 Apr;34(4):433-8.
Heterogenous biological character of myeloma cells was associated with different expression of adhesion molecules. Myeloma cells could be phenotypically divided into two subpopulations: CD38++/VLA5+/MPC-1+(VLA-5+) cells and CD38++/VLA5-/MPC-1-(VLA-5-) cells. VLA-5- myeloma cells were morphologically immature and proliferated markedly with response to IL-6 in vitro, while VLA-5+ cells showed very low uptakes of 3H-TdR but secreted higher amounts of M-protein in vitro. These results suggest VLA-5- cells are proliferative precursor in myeloma. With respect to VLA-5 and MPC-1 expression, myeloma precursor cells (CD38++/VLA-5-/MPC-1-/CD10-/CD24-) showed similar phenotype to germinal center B cells (CD38+/VLA-5-/MPC-1-/CD10+/CD24-), rather than that of pre-B cells in the bone marrow (CD38+/VLA-5+/MPC-1-/CD10+/CD24+). Identification of precursor cells and characterization of their growth is important for the understanding of pathophysiology of myeloma and the therapeutic strategy.
骨髓瘤细胞的异质性生物学特性与黏附分子的不同表达相关。骨髓瘤细胞在表型上可分为两个亚群：CD38++/VLA5+/MPC-1+(VLA-5+)细胞和CD38++/VLA5-/MPC-1-(VLA-5-)细胞。VLA-5-骨髓瘤细胞形态不成熟，在体外对IL-6反应时显著增殖，而VLA-5+细胞体外3H-TdR摄取量极低，但分泌较高量的M蛋白。这些结果表明VLA-5-细胞是骨髓瘤中的增殖前体细胞。关于VLA-5和MPC-1表达，骨髓瘤前体细胞(CD38++/VLA-5-/MPC-1-/CD10-/CD24-)表现出与生发中心B细胞(CD38+/VLA-5-/MPC-1-/CD10+/CD24-)相似的表型，而非骨髓中前B细胞(CD38+/VLA-5+/MPC-1-/CD10+/CD24+)的表型。前体细胞的鉴定及其生长特性的表征对于理解骨髓瘤的病理生理学和治疗策略很重要。
