利用SOS色变试验对铝等离子体蚀刻废品进行遗传毒性评估。

Genotoxicity assessment of waste products of aluminum plasma etching with the SOS chromotest.

作者信息

Raabe F, Janz S, Wolff G, Merten H, Landrock A, Birkenfeld T, Herzschuh R

机构信息

Institute of Clinical Immunology, Faculty of Medicine, University Leipzig, Germany.

出版信息

Mutat Res. 1993 Jul;300(2):99-109. doi: 10.1016/0165-1218(93)90127-y.

DOI:10.1016/0165-1218(93)90127-y

PMID:7685499

Abstract

We evaluated 36 characteristic waste products from the plasma etching of aluminum for genotoxicity with the SOS chromotest. The majority of the samples showed genotoxic activity in tester strain Escherichia coli PQ37 without metabolic activation using S9 mix. In the presence of S9, a deactivation of the samples was regularly observed. Comparable studies with the Salmonella/microsome (Ames) test using tester strains Salmonella typhimurium TA98 and TA100 indicated actual mutagenicity of waste products. Gas chromatograms of the organic constituents of all waste products were performed in parallel with the genotoxicity assays. In contrast to the similarity of the peak patterns of all chromatograms, the biological effects of individual waste samples showed large differences. Information on chemical composition and the SOS chromotest results of a representative sample recovered over a period of 2 years is given. For this sample, the influences of sample preparation and cytotoxic matrix effects on the test parameters are also shown.

摘要

我们用SOS显色试验评估了36种铝等离子体蚀刻产生的典型废品的遗传毒性。大多数样品在不使用S9混合物进行代谢活化的情况下，在测试菌株大肠杆菌PQ37中显示出遗传毒性活性。在有S9存在的情况下，经常观察到样品失活。使用测试菌株鼠伤寒沙门氏菌TA98和TA100进行的沙门氏菌/微粒体（艾姆斯）试验的类似研究表明废品具有实际致突变性。所有废品有机成分的气相色谱图与遗传毒性试验同时进行。与所有色谱图峰型的相似性相反，各个废品样品的生物学效应显示出很大差异。给出了在两年时间内回收的一个代表性样品的化学成分信息和SOS显色试验结果。对于该样品，还展示了样品制备和细胞毒性基质效应对测试参数的影响。