Henderson J E, Soderlund D M, Knipple D C
New York State Agricultural Experiment Station, Cornell University, Geneva 14456.
Biochem Biophys Res Commun. 1993 Jun 15;193(2):474-82. doi: 10.1006/bbrc.1993.1648.
A cDNA encoding a novel member of the ligand-gated chloride channel gene family of insects has been isolated from Drosophila melanogaster and characterized. The 1488 nucleotide open reading frame of this cDNA encodes an amino acid sequence having structural features conserved among ligand-gated chloride channel subunit proteins, including four hydrophobic domains capable of forming transmembrane helices (M1-M4), an octapeptide "signature motif" occurring in M2, a large intracellular domain between M3 and M4, and cysteine residues postulated to form a disulfide-bridged loop structure in the extracellular domain. Among characterized members of this gene family, this gene exhibits the greatest similarity to GABA receptor beta subunit genes. This high level of structural similarity suggests that its encoded protein may be a functional homologue of the beta subunit family in the D. melanogaster nervous system.
从黑腹果蝇中分离并鉴定了一个编码昆虫配体门控氯离子通道基因家族新成员的cDNA。该cDNA的1488个核苷酸开放阅读框编码一个氨基酸序列，该序列具有配体门控氯离子通道亚基蛋白中保守的结构特征，包括四个能够形成跨膜螺旋的疏水结构域（M1-M4）、M2中出现的八肽“特征基序”、M3和M4之间的一个大的细胞内结构域，以及推测在细胞外结构域形成二硫键桥环结构的半胱氨酸残基。在该基因家族已鉴定的成员中，该基因与GABA受体β亚基基因表现出最大的相似性。这种高度的结构相似性表明，其编码的蛋白质可能是黑腹果蝇神经系统中β亚基家族的功能同源物。
