Vizzard M A, Erdman S L, de Groat W C
Department of Pharmacology, University of Pittsburgh, School of Medicine, PA 15261.
Neurosci Lett. 1993 Apr 2;152(1-2):72-6. doi: 10.1016/0304-3940(93)90486-5.
The NADPH diaphorase histochemical reaction was used in combination with retrograde axonal transport of Fluorogold (FG) from the major pelvic ganglion (MPG) to determine if NADPH diaphorase is contained within afferent and preganglionic efferent pathways to pelvic visceral organs. In L6 and S1 dorsal root ganglia, 68.5% and 62.2%, respectively, of FG-labeled afferent neurons were NADPH-diaphorase positive. In the sacral parasympathetic nucleus (SPN) of the L6 and S1 spinal cord segments, 49.4% and 51.7%, respectively of FG labeled preganglionic efferent neurons were NADPH-diaphorase positive. NADPH-diaphorase-positive neurons were also observed in laminae I and II of the dorsal horn, around the central canal and in the dorsal commissure. In addition, fiber-like NADPH diaphorase staining was present in superficial dorsal horn, Lissauer's tract and the lateral edge of the dorsal horn extending into the region of the SPN. If NADPH diaphorase activity in neurons does indicate a physiological function of nitric oxide, then nitric oxide may have a role as a neuromodulator or neurotransmitter within visceral afferent and preganglionic efferent pathways to the pelvic viscera in the rat.
采用还原型辅酶Ⅱ黄递酶组织化学反应，结合荧光金（FG）从主要盆神经节（MPG）进行的逆行轴突运输，以确定盆内脏器的传入和节前传出通路中是否含有还原型辅酶Ⅱ黄递酶。在L6和S1背根神经节中，分别有68.5%和62.2%的FG标记传入神经元为还原型辅酶Ⅱ黄递酶阳性。在L6和S1脊髓节段的骶副交感核（SPN）中，分别有49.4%和51.7%的FG标记节前传出神经元为还原型辅酶Ⅱ黄递酶阳性。在背角的Ⅰ层和Ⅱ层、中央管周围以及背连合中也观察到了还原型辅酶Ⅱ黄递酶阳性神经元。此外，在背角浅层、Lissauer束以及延伸至SPN区域的背角外侧边缘存在纤维状还原型辅酶Ⅱ黄递酶染色。如果神经元中的还原型辅酶Ⅱ黄递酶活性确实表明一氧化氮具有生理功能，那么一氧化氮可能在大鼠盆内脏器的内脏传入和节前传出通路中作为一种神经调节剂或神经递质发挥作用。
