Vizzard M A, Erdman S L, de Groat W C
University of Pittsburgh, School of Medicine, Department of Pharmacology, Pennsylvania 15261.
J Auton Nerv Syst. 1993 Jul;44(1):85-90. doi: 10.1016/0165-1838(93)90382-5.
NADPH diaphorase histochemistry was used in combination with axonal labelling techniques to determine if NADPH diaphorase is present in afferent and postganglionic efferent pathways to the urinary bladder of the rat. In the L6 and S1 dorsal root ganglia, 80.9 and 78.5%, respectively, of bladder afferent neurons labelled with fluorescent dyes were NADPH diaphorase positive. In the major pelvic ganglion (MPG), many non-labelled neurons and fibers were intensely stained for NADPH diaphorase. Intensely stained cells were clustered near the exit of the penile nerve although stained cells were also scattered throughout the ganglion. Only a small percentage (3.5%) of bladder postganglionic neurons in the MPG were NADPH diaphorase positive. Since NADPH diaphorase activity commonly reflects the presence of nitric oxide synthase, the present findings raise the possibility that nitric oxide may have a role as a neurotransmitter or neuromodulator in afferent pathways from the urinary bladder.
采用还原型辅酶Ⅱ黄递酶组织化学法并结合轴突标记技术，以确定大鼠膀胱传入和节后传出通路中是否存在还原型辅酶Ⅱ黄递酶。在L6和S1背根神经节中，分别有80.9%和78.5%用荧光染料标记的膀胱传入神经元为还原型辅酶Ⅱ黄递酶阳性。在主要盆神经节（MPG）中，许多未标记的神经元和纤维被强烈染为还原型辅酶Ⅱ黄递酶阳性。尽管染色细胞也散布于整个神经节，但强烈染色的细胞聚集在阴茎神经出口附近。MPG中仅一小部分（3.5%）膀胱节后神经元为还原型辅酶Ⅱ黄递酶阳性。由于还原型辅酶Ⅱ黄递酶活性通常反映一氧化氮合酶的存在，目前的研究结果提示，一氧化氮可能在膀胱传入通路中作为神经递质或神经调质发挥作用。
