Ensari A, Ager A, Marsh M N, Morgan S, Moriarty K
University Department of Medicine, University of Manchester School of Medicine, Hope Hospital, Salford, UK.
Agents Actions. 1993;38 Spec No:C47-50. doi: 10.1007/BF01991133.
Expression of endothelial adhesion molecules is a key factor in localizing inflammatory processes. We have evaluated the time-course expression of E-selectin, ICAM-1, and VCAM-1 in rectal mucosae from coeliac patients (CD) with T-lymphocyte sensitization to wheat (gliadin) protein. Ten treated, eight untreated CD patients and five disease controls underwent a dynamic 4 h rectal gluten challenge. Seven treated CD patients had a 24 h challenge to evaluate the time-course changes. Coeliac tissue significantly increased VCAM-1 expression [p < 0.001 (untreated and treated CD)] and E-selectin [p = 0.01 (untreated CD only)]-expression compared to controls but not ICAM-1. VCAM-1 continued to increase up to 24 h, while E-selectin decreased after 4 h. These findings relate to a 8 h rise in CD3 (+) lymphocytes [p < 0.05 (treated)] and a 4 h rise in neutrophils (p < 0.005). This approach permits a novel study of the inflammatory response to a known immunogen in human patients.
内皮黏附分子的表达是炎症过程定位的关键因素。我们评估了对小麦（麦醇溶蛋白）蛋白有T淋巴细胞致敏的乳糜泻患者（CD）直肠黏膜中E-选择素、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的时间进程表达。10名接受治疗的、8名未接受治疗的CD患者以及5名疾病对照者接受了长达4小时的直肠麸质激发试验。7名接受治疗的CD患者进行了24小时激发试验以评估时间进程变化。与对照组相比，乳糜泻组织中VCAM-1表达显著增加[p < 0.001（未治疗和治疗后的CD）]，E-选择素表达也显著增加[p = 0.01（仅未治疗的CD）]，但ICAM-1未增加。VCAM-1持续增加至24小时，而E-选择素在4小时后下降。这些发现与CD3(+)淋巴细胞增加8小时[p < 0.05（治疗后）]和中性粒细胞增加4小时（p < 0.005）有关。这种方法允许对人类患者中对已知免疫原的炎症反应进行新的研究。
