Bandaletova T, Brouet I, Bartsch H, Sugimura T, Esumi H, Ohshima H
Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer, Lyon, France.
APMIS. 1993 Apr;101(4):330-6.
Immunohistochemical localization of an endotoxin-inducible form of nitric oxide synthase was examined using rabbit polyclonal antibody against the enzyme purified from rat liver. In rats treated with Propionibacterium acnes and lipopolysaccharide, immunostaining was observed in macrophages, occasional lymphocytes, neutrophils and eosinophils in red pulp of spleen, Kupffer cells, endothelial cells and hepatocytes in liver, alveolar macrophages in lung, macrophages and endothelial cells in adrenal glands, and histiocytes, eosinophils, mast cells and endothelial cells in colon. Immunoreactivity was also evident in the following tissues: histiocytes and endothelial cells in kidney; histiocytes and neutrophils in esophagus; macrophages and eosinophils in duodenum; macrophages, some lymphocytes and mast cells in ileum; histiocytes in thymus; and endothelial cells in heart and aorta. Immunoreactivity was not detected in these organs from untreated rats. Positively staining cells in these rat organs appeared within 2.5 h after lipopolysaccharide administration; their number dramatically increased within the next 2.5 h, remained at high levels for a further 19 h, and then decreased over the following 24 h. The number of positive cells appearing correlated well with the nitric oxide synthase activity biochemically determined in the same organs.
使用针对从大鼠肝脏纯化的该酶的兔多克隆抗体，检测了内毒素诱导型一氧化氮合酶的免疫组织化学定位。在用痤疮丙酸杆菌和脂多糖处理的大鼠中，在脾脏红髓中的巨噬细胞、偶尔的淋巴细胞、中性粒细胞和嗜酸性粒细胞、肝脏中的库普弗细胞、内皮细胞和肝细胞、肺中的肺泡巨噬细胞、肾上腺中的巨噬细胞和内皮细胞以及结肠中的组织细胞、嗜酸性粒细胞、肥大细胞和内皮细胞中观察到免疫染色。在以下组织中免疫反应性也很明显：肾脏中的组织细胞和内皮细胞；食管中的组织细胞和中性粒细胞；十二指肠中的巨噬细胞和嗜酸性粒细胞；回肠中的巨噬细胞、一些淋巴细胞和肥大细胞；胸腺中的组织细胞；以及心脏和主动脉中的内皮细胞。在未处理大鼠的这些器官中未检测到免疫反应性。这些大鼠器官中的阳性染色细胞在给予脂多糖后2.5小时内出现；其数量在接下来的2.5小时内急剧增加，在接下来的19小时内保持在高水平，然后在接下来的24小时内下降。出现的阳性细胞数量与在相同器官中生化测定的一氧化氮合酶活性密切相关。
