Carey D G, Iismaa T P, Ho K Y, Rajkovic I A, Kelly J, Kraegen E W, Ferguson J, Inglis A S, Shine J, Chisholm D J
Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, New South Wales, Australia.
J Clin Endocrinol Metab. 1993 Jul;77(1):90-3. doi: 10.1210/jcem.77.1.7686918.
Human galanin (hGAL) is a 30-amino acid neurohormone that has recently been shown to differ significantly from porcine and rat GAL. We investigated the endocrine and cardiovascular effects of hGAL in eight male volunteers. On three separate occasions, each received a 90-min infusion of saline, low dose (33 pmol/kg.min) and high (132 pmol/kg.min) dose hGAL, combined with an iv glucose bolus (to assess effects on insulin and GH release). hGAL was undetectable, 1.4 +/- 0.2 nmol/L, and 3.7 +/- 0.5 nmol/L during control, low dose, and high dose studies, respectively. The half-life of hGAL was 3.5 +/- 0.5 min. GH levels rose significantly in both studies (vs. control) and were not suppressed by hyperglycemia [low dose area under the curve (AUC), 1827 +/- 348 micrograms/min.L (P < 0.05); peak, 19.5 +/- 5.3 micrograms/L; high dose AUC, 1896 +/- 401 micrograms/min.L (P < 0.005); peak, 28.0 +/- 7.5 micrograms/L]. PRL levels rose significantly with the high dose study only (AUC, 12.8 +/- 1.1 micrograms/min.L; P < 0.01). FSH, LH, and catecholamine levels were unchanged. Glucose-stimulated insulin release was not inhibited. There was a dose-dependent increase in pulse rate and a profound decrease in sinus arrhythmia, but no change in blood pressure. These cardiovascular effects have not been reported with studies in humans using GAL of other species. We conclude that hGAL may play an important role in man in modulating GH secretion and cardiac vagal tone, but not insulin release.
人甘丙肽（hGAL）是一种由30个氨基酸组成的神经激素，最近研究表明其与猪和大鼠甘丙肽有显著差异。我们对8名男性志愿者进行了hGAL的内分泌和心血管效应研究。在三个不同时间，每位志愿者分别接受90分钟的生理盐水输注、低剂量（33 pmol/kg·min）和高剂量（132 pmol/kg·min）hGAL输注，并静脉注射葡萄糖推注（以评估对胰岛素和生长激素释放的影响）。在对照、低剂量和高剂量研究期间，hGAL水平分别不可检测、1.4±0.2 nmol/L和3.7±0.5 nmol/L。hGAL的半衰期为3.5±0.5分钟。两项研究中生长激素水平均显著升高（与对照相比），且未被高血糖抑制[低剂量曲线下面积（AUC），1827±348微克/分钟·升（P<0.05）；峰值，19.5±5.3微克/升；高剂量AUC，1896±401微克/分钟·升（P<0.005）；峰值，28.0±7.5微克/升]。仅在高剂量研究中催乳素水平显著升高（AUC，12.8±1.1微克/分钟·升；P<0.01）。促卵泡激素、促黄体生成素和儿茶酚胺水平未改变。葡萄糖刺激的胰岛素释放未受抑制。心率呈剂量依赖性增加，窦性心律失常显著减少，但血压无变化。使用其他物种甘丙肽对人类进行的研究尚未报道这些心血管效应。我们得出结论，hGAL在调节人类生长激素分泌和心脏迷走神经张力方面可能起重要作用，但对胰岛素释放无作用。
