Ranson R N, Butler P J, Taylor E W
School of Biological Sciences, University of Birmingham, UK.
J Auton Nerv Syst. 1993 May;43(2):123-37. doi: 10.1016/0165-1838(93)90349-y.
The location of vagal preganglionic neurones (VPN) has been determined in nine ferrets (Mustela putorius furo) and seven mink (M. vison) using neuronal tract-tracing techniques employing horseradish peroxidase (HRP) and wheat-germ agglutinin conjugated HRP (WGA-HRP) mixtures injected into the nodose ganglion of the vagus nerve. Labelled VPN were located ipsilaterally in the dorsal motor nucleus of the vagus (DmnX), nucleus ambiguus (nA), and reticular formation (rf) of the medulla oblongata. In four of the ferrets, labelled VPN were also identified in the nucleus dorsomedialis (ndm) and the nucleus of the spinal accessory nerve (nspa). In a single mink a few labelled cells were observed in the ndm but no labelled VPN were found in the nspa. Labelling of afferent components of the vagus nerve was seen in two ferrets and two mink with the best labelling obtained following an injection of an HRP/WGA-HRP mixture into the nodose ganglion. Labelled afferents were observed to cross the ipsilateral spinal trigeminal tract (SpV) before entering the tractus solitarius (TS) in regions separate from the motor axons which exit the medulla in separate fasicles. Sensory terminal fields were identified bilaterally in the nucleus of the tractus solitarius (nTS) in both species and bilaterally in the area postrema (ap) of the ferret; however, the contralateral labelling was sparse in comparison to the densely labelled ipsilateral nTS/ap. Maximal terminal labelling was seen in regions just rostral and caudal to obex in both species.
利用将辣根过氧化物酶（HRP）和小麦胚芽凝集素结合的HRP（WGA-HRP）混合物注入迷走神经结状神经节的神经束追踪技术，已确定了9只雪貂（鼬属黑足鼬）和7只水貂（鼬属美洲水鼬）中迷走神经节前神经元（VPN）的位置。标记的VPN位于延髓迷走神经背运动核（DmnX）、疑核（nA）和网状结构（rf）的同侧。在4只雪貂中，还在背内侧核（ndm）和脊髓副神经核（nspa）中发现了标记的VPN。在1只水貂中，在ndm中观察到少量标记细胞，但在nspa中未发现标记的VPN。在2只雪貂和2只水貂中观察到了迷走神经传入成分的标记，将HRP/WGA-HRP混合物注入结状神经节后获得了最佳标记。观察到标记的传入纤维在进入孤束（TS）之前穿过同侧脊髓三叉神经束（SpV），其区域与以单独束状离开延髓的运动轴突分开。在两个物种的孤束核（nTS）双侧以及雪貂的最后区（ap）双侧都识别出了感觉终末野；然而，与同侧nTS/ap的密集标记相比，对侧标记稀疏。在两个物种中，在闩前方和后方的区域观察到了最大的终末标记。
