Nelson J W, Butler S L, Brown P H, Greening A P, Govan J R
Department of Medical Microbiology, University of Edinburgh Medical School.
J Med Microbiol. 1993 Jul;39(1):39-47. doi: 10.1099/00222615-39-1-39.
The immunological response of cystic fibrosis (CF) patients to lipopolysaccharide (LPS) antigens of Pseudomonas cepacia was investigated. Enzyme-linked immunosorbent assays (ELISA) with either P. cepacia whole cells or extracted core LPS from a clinical isolate of P. cepacia as antigen were used to measure serum IgG and sputum IgA anti-P. cepacia antibodies. The ELISA with core LPS distinguished nine CF patients colonised by P. cepacia from nine age- and sex-matched non-colonised CF patients. The rate of increase of anti-P. cepacia IgG antibodies after bacteriologically proven P. cepacia colonisation varied in individual patients: in some patients the first isolation of P. cepacia was preceded or accompanied by a two-to-four-fold rise in anti-P. cepacia LPS IgG titres. Absorption studies and immunoblot analysis of serum from patients colonised with P. cepacia demonstrated that a significant component of the anti-P. cepacia core LPS antibodies was specific for P. cepacia and did not react with the core LPS of P. aeruginosa. Immunoblotting also illustrated that there may be a degree of core heterogeneity between different isolates of P. cepacia. Detection of P. cepacia LPS specific antibodies in serum (IgG) and sputum (IgA) from CF patients is recommended to assist the identification of P. cepacia colonisation in CF patients.
对囊性纤维化（CF）患者针对洋葱伯克霍尔德菌脂多糖（LPS）抗原的免疫反应进行了研究。采用以洋葱伯克霍尔德菌全细胞或从一株临床分离的洋葱伯克霍尔德菌中提取的核心LPS作为抗原的酶联免疫吸附测定（ELISA），来检测血清IgG和痰液IgA抗洋葱伯克霍尔德菌抗体。以核心LPS进行的ELISA能够区分9例被洋葱伯克霍尔德菌定植的CF患者与9例年龄和性别匹配的未被定植的CF患者。在经细菌学证实的洋葱伯克霍尔德菌定植后，抗洋葱伯克霍尔德菌IgG抗体的升高速率在个体患者中有所不同：在一些患者中，首次分离出洋葱伯克霍尔德菌之前或同时，抗洋葱伯克霍尔德菌LPS IgG滴度会升高2至4倍。对被洋葱伯克霍尔德菌定植患者的血清进行吸收研究和免疫印迹分析表明，抗洋葱伯克霍尔德菌核心LPS抗体的一个重要成分对洋葱伯克霍尔德菌具有特异性，且不与铜绿假单胞菌的核心LPS发生反应。免疫印迹还表明，不同的洋葱伯克霍尔德菌分离株之间可能存在一定程度的核心异质性。建议检测CF患者血清（IgG）和痰液（IgA）中洋葱伯克霍尔德菌LPS特异性抗体，以协助识别CF患者中的洋葱伯克霍尔德菌定植情况。
