Butler A, Tsunoda S, McCobb D P, Wei A, Salkoff L
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Science. 1993 Jul 9;261(5118):221-4. doi: 10.1126/science.7687074.
Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.
从小鼠脑和骨骼肌中分离出编码钙激活钾通道的基因mSlo的互补DNA（cDNA），进行测序，并在非洲爪蟾卵母细胞中表达。mSlo编码的通道类似于“大电导”或BK（高电导）通道类型；在钾离子浓度对称的情况下，单通道电导为272皮西门子。全细胞电流和单通道电流被蝎毒素、iberiotoxin和四乙铵离子阻断。分离出了大量变异的mSlo cDNA，表明单一基因可产生几种不同的哺乳动物BK通道类型。
