二乙基亚硝胺和部分肝切除诱导大鼠肝脏中α2u球蛋白mRNA水平降低。
Diethylnitrosamine- and partial hepatectomy-induced decrease in alpha 2u-globulin mRNA level in the rat liver.
作者信息
Matuoka K, Markus I, Wong A, Smith G J
机构信息
Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Japan.
出版信息
J Cancer Res Clin Oncol. 1993;119(10):572-5. doi: 10.1007/BF01372719.
Changes in gene expression in the rat liver were investigated by analyzing cDNA libraries for liver mRNAs from adult male rats injected with a chemical carcinogen diethylnitrosamine (DEN). Differential screening using normal and DEN-treated liver cDNAs as probes demonstrated that some of the mRNA species had noticeably lower abundance in the DEN-treated liver than in the untreated liver. Surprisingly, most of those clones were found to code for alpha 2u-globulin (A2uG), an abundant protein in the male rat liver. Further analysis by in situ hybridization revealed that the decrease in the A2uG mRNA level occurred in the area where liver cells were proliferating due to DEN treatment and/or partial hepatectomy (PH). The findings indicate coincidence of cell proliferation with a decrease in the A2uG gene expression in the adult male rat liver, implying that the A2uG-related change favors chemical carcinogen-induced cell growth.
通过分析注射化学致癌物二乙基亚硝胺（DEN）的成年雄性大鼠肝脏mRNA的cDNA文库，研究了大鼠肝脏中的基因表达变化。使用正常肝脏和经DEN处理的肝脏cDNA作为探针进行差异筛选，结果表明，某些mRNA种类在经DEN处理的肝脏中的丰度明显低于未处理的肝脏。令人惊讶的是，发现这些克隆中的大多数编码α2u球蛋白（A2uG），这是雄性大鼠肝脏中的一种丰富蛋白质。通过原位杂交进一步分析发现，A2uG mRNA水平的降低发生在由于DEN处理和/或部分肝切除术（PH）导致肝细胞增殖的区域。这些发现表明成年雄性大鼠肝脏中细胞增殖与A2uG基因表达降低同时发生，这意味着与A2uG相关的变化有利于化学致癌物诱导的细胞生长。
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