Peehl D M, Wong S T, Stamey T A
Department of Urology, Stanford University School of Medicine, CA 94305-5118.
Prostate. 1993;23(1):69-78. doi: 10.1002/pros.2990230107.
The response of cultured human prostatic epithelial cells to vitamin A was measured by clonal growth assay in serum-free medium. Retinoic acid at 3 nM or higher inhibited the proliferation of cell strains derived from normal, benign hyperplastic and malignant tissues, while lower levels (0.03 nM) were stimulatory. Reduced proliferation induced by retinoic acid was accompanied by a marked change in morphology, as intercellular adhesion decreased. In conjunction, the expression of keratins 8 and 18, associated with the differentiated luminal phenotype of prostatic epithelia, was increased. In post-confluent cultures, retinoic acid prevented the appearance of keratin 1, which accompanied the development of a squamous phenotype by cells maintained under these conditions. The findings of this study indicate a role for vitamin A as a modulator of the growth and differentiation of prostatic epithelial cells.
在无血清培养基中通过克隆生长测定法测量培养的人前列腺上皮细胞对维生素A的反应。3 nM或更高浓度的视黄酸抑制了源自正常、良性增生和恶性组织的细胞株的增殖，而较低浓度（0.03 nM）则具有刺激作用。视黄酸诱导的增殖减少伴随着形态的显著变化，因为细胞间粘附减少。同时，与前列腺上皮细胞分化的管腔表型相关的角蛋白8和18的表达增加。在汇合后培养中，视黄酸可防止角蛋白1的出现，而在这些条件下培养的细胞会出现鳞状表型。本研究结果表明维生素A作为前列腺上皮细胞生长和分化的调节剂发挥作用。
