Peehl D M, Wong S T, Rubin J S
Department of Urology, Stanford University School of Medicine, California 94305, USA.
Growth Regul. 1996 Mar;6(1):22-31.
Previous studies indicate that keratinocyte growth factor (KGF) acts as a paracrine factor in the prostatic epithelium and epidermal growth factor (EGF) acts as an autocrine factor. In serum-free medium, KGF or EGF promoted similar growth of human prostatic epithelial cells. Response to two growth-inhibitory factors (suramin and transforming growth factor-beta), and expression of keratins and prostate-specific antigen (PSA), were similar with either mitogen. However, colonies in medium with KGF were very compact with extensive intercellular bonds, whereas colonies with EGF consisted of widely-separated cells. Growth was decreased to a greater extent by deletion of growth factors from medium with KGF versus EGF, and retinoic acid was 10-fold more potent at inducing growth inhibition and differentiation-associated keratin with KGF compared with EGF. We conclude that regulation of growth and differentiation in the prostate might vary depending on the availability of KGF versus EGF.
先前的研究表明，角质形成细胞生长因子（KGF）在前列腺上皮中作为旁分泌因子起作用，而表皮生长因子（EGF）作为自分泌因子起作用。在无血清培养基中，KGF或EGF促进人前列腺上皮细胞的相似生长。对两种生长抑制因子（苏拉明和转化生长因子-β）的反应以及角蛋白和前列腺特异性抗原（PSA）的表达，在两种有丝分裂原作用下相似。然而，含有KGF的培养基中的集落非常紧密，细胞间连接广泛，而含有EGF的集落由分散的细胞组成。与EGF相比，从含有KGF的培养基中去除生长因子时，生长下降的程度更大，并且视黄酸在与KGF一起诱导生长抑制和分化相关角蛋白方面的效力是与EGF一起时的10倍。我们得出结论，前列腺中生长和分化的调节可能因KGF与EGF的可获得性而异。
