Coursaget P, Buisson Y, Depril N, le Cann P, Chabaud M, Molinié C, Roue R
Institut de Virologie de Tours, Faculté de Pharmacie, France.
FEMS Microbiol Lett. 1993 May 15;109(2-3):251-5. doi: 10.1111/j.1574-6968.1993.tb06176.x.
Hepatitis E virus (HEV) is the causative agent of non-A, non-B hepatitis which is transmitted by the fecal-oral route and occurs principally in the form of large epidemics and outbreaks in developing countries. Two overlapping synthetic peptides corresponding to overlapping DNA sequences of the ORF 3 of HEV genome were found to be immunoreactive with sera from patients involved in two epidemics of enterically transmitted non-A, non-B hepatitis. The results suggested the existence of two distinct epitopes. The four synthetic peptides representing these two epitopes from Burma and Mexico strains of hepatitis E virus, were used to investigate anti-HEV reactivities. HEV antibodies were detected in 84-88% of HEV-infected individuals according to the peptide used. The results suggest that a peptide-based ELISA can provide an accurate tool for the diagnosis of acute hepatitis type E.
戊型肝炎病毒（HEV）是非甲非乙型肝炎的病原体，通过粪-口途径传播，主要以大规模流行和暴发的形式在发展中国家出现。发现与HEV基因组ORF 3重叠DNA序列相对应的两个重叠合成肽与参与两起经肠道传播的非甲非乙型肝炎流行的患者血清发生免疫反应。结果提示存在两个不同的表位。使用代表来自缅甸和墨西哥戊型肝炎病毒株这两个表位的四种合成肽来研究抗HEV反应性。根据所使用的肽，在84%-88%的HEV感染个体中检测到了HEV抗体。结果表明基于肽的ELISA可为急性戊型肝炎的诊断提供一种准确的工具。
