具有戊型肝炎病毒表位的嵌合乙型肝炎病毒核心颗粒的杆状病毒表达及其在戊型肝炎免疫测定中的应用。
Baculovirus expression of chimeric hepatitis B virus core particles with hepatitis E virus epitopes and their use in a hepatitis E immunoassay.
作者信息
Touze A, Enogat N, Buisson Y, Coursaget P
机构信息
Institut de Virologie de Tours and Laboratoire des Processus Infectieux et Tumoraux, Faculté des Sciences Pharmaceutiques "Philippe Maupas," 37200 Tours, France.
出版信息
J Clin Microbiol. 1999 Feb;37(2):438-41. doi: 10.1128/JCM.37.2.438-441.1999.
Abstract
Two hepatitis B core proteins bearing the immunodominant region of the hepatitis E virus (HEV) capsid protein, one at the C terminus of hepatitis B virus core antigen (HBcAg) and the other within the HBcAg immunodominant loop, were constructed. Both chimeric proteins exhibited HEV reactivity, but only the first construct retained HBcAg reactivity. The second construct was used to develop an anti-HEV test which is equivalent to a commercial test for the detection of anti-HEV immunoglobulin G (IgG) but is more sensitive for the detection of anti-HEV IgM.
摘要
构建了两种带有戊型肝炎病毒（HEV）衣壳蛋白免疫显性区的乙型肝炎核心蛋白，一种位于乙型肝炎病毒核心抗原（HBcAg）的C末端，另一种位于HBcAg免疫显性环内。两种嵌合蛋白均表现出对HEV的反应性，但只有第一种构建体保留了对HBcAg的反应性。第二种构建体用于开发一种抗HEV检测方法，该方法等同于用于检测抗HEV免疫球蛋白G（IgG）的商业检测方法，但对抗HEV IgM的检测更敏感。
相似文献
1
Baculovirus expression of chimeric hepatitis B virus core particles with hepatitis E virus epitopes and their use in a hepatitis E immunoassay.具有戊型肝炎病毒表位的嵌合乙型肝炎病毒核心颗粒的杆状病毒表达及其在戊型肝炎免疫测定中的应用。
J Clin Microbiol. 1999 Feb;37(2):438-41. doi: 10.1128/JCM.37.2.438-441.1999.
2
IgM and IgG antibodies to hepatitis E virus (HEV) detected by an enzyme immunoassay based on an HEV-specific artificial recombinant mosaic protein.通过基于戊型肝炎病毒（HEV）特异性人工重组嵌合蛋白的酶免疫测定法检测到的抗戊型肝炎病毒（HEV）IgM和IgG抗体。
J Med Virol. 1996 Sep;50(1):50-8. doi: 10.1002/(SICI)1096-9071(199609)50:1<50::AID-JMV10>3.0.CO;2-1.
3
Detection of immunoglobulin M antibodies to hepatitis E virus by class capture enzyme immunoassay.通过类捕获酶免疫测定法检测戊型肝炎病毒免疫球蛋白M抗体
Clin Diagn Lab Immunol. 2003 Jul;10(4):579-86. doi: 10.1128/cdli.10.4.579-586.2003.
4
Diagnostic performance of selected commercial HEV IgM and IgG ELISAs for immunocompromised and immunocompetent patients.用于免疫功能低下和免疫功能正常患者的选定商业 HEV IgM 和 IgG ELISA 的诊断性能。
J Clin Virol. 2013 Dec;58(4):629-34. doi: 10.1016/j.jcv.2013.10.010. Epub 2013 Oct 17.
5
Empty virus-like particle-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus.基于空病毒样颗粒的戊型肝炎病毒抗体酶联免疫吸附测定
J Med Virol. 2000 Nov;62(3):327-33. doi: 10.1002/1096-9071(200011)62:3<327::aid-jmv4>3.0.co;2-1.
6
Evaluation of human (genotype 1) and swine (genotype 4)-ORF2-based ELISAs for anti-HEV IgM and IgG detection in an endemic country and search for type 4 human HEV infections.在一个地方流行国家评估基于人（1型基因型）和猪（4型基因型）开放阅读框2（ORF2）的酶联免疫吸附测定（ELISA）检测抗戊型肝炎病毒（HEV）IgM和IgG，并寻找4型人HEV感染情况。
J Viral Hepat. 2007 Jun;14(6):435-45. doi: 10.1111/j.1365-2893.2006.00801.x.
7
Clinical significance of anti-HEV IgA in diagnosis of acute genotype 4 hepatitis E virus infection negative for anti-HEV IgM.抗戊型肝炎病毒IgA在抗戊型肝炎病毒IgM阴性的急性4型戊型肝炎病毒感染诊断中的临床意义
Dig Dis Sci. 2009 Nov;54(11):2512-8. doi: 10.1007/s10620-008-0657-4. Epub 2009 Jan 1.
8
Production in Pichia pastoris and characterization of genetic engineered chimeric HBV/HEV virus-like particles.毕赤酵母中基因工程嵌合型乙肝病毒/戊肝病毒样颗粒的生产及特性研究
Chin Med Sci J. 2004 Jun;19(2):78-83.
9
Comparative sensitivity of commercial tests for hepatitis E genotype 3 virus antibody detection.商业检测方法检测戊型肝炎病毒 3 型抗体的比较敏感性。
J Med Virol. 2015 Nov;87(11):1934-9. doi: 10.1002/jmv.24251. Epub 2015 May 29.
10
Acute sporadic hepatitis E in children: diagnostic relevance of specific immunoglobulin M and immunoglobulin G compared with nested reverse transcriptase PCR.儿童急性散发性戊型肝炎：特异性免疫球蛋白M和免疫球蛋白G与巢式逆转录聚合酶链反应相比的诊断相关性
FEMS Immunol Med Microbiol. 2006 Oct;48(1):16-20. doi: 10.1111/j.1574-695X.2006.00111.x.
引用本文的文献
1
Antibody levels against BK virus and prostate, kidney and bladder cancers in the EPIC-Oxford cohort.欧洲癌症与营养前瞻性调查（EPIC）牛津队列中针对BK病毒以及前列腺癌、肾癌和膀胱癌的抗体水平。
Br J Cancer. 2005 Nov 28;93(11):1305-6. doi: 10.1038/sj.bjc.6602869.
2
Assembly of human severe acute respiratory syndrome coronavirus-like particles.人严重急性呼吸综合征冠状病毒样颗粒的组装。
Biochem Biophys Res Commun. 2004 Jun 11;318(4):833-8. doi: 10.1016/j.bbrc.2004.04.111.
3
Clinical and epidemiological relevance of quantitating hepatitis E virus-specific immunoglobulin M.戊型肝炎病毒特异性免疫球蛋白M定量的临床及流行病学相关性
Clin Diagn Lab Immunol. 2002 Sep;9(5):1072-8. doi: 10.1128/cdli.9.5.1072-1078.2002.
4
Quantitation of immunoglobulin to hepatitis E virus by enzyme immunoassay.采用酶免疫测定法定量检测抗戊型肝炎病毒免疫球蛋白
Clin Diagn Lab Immunol. 2002 May;9(3):639-48. doi: 10.1128/cdli.9.3.639-648.2002.
5
Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions.通过抑制人乳头瘤病毒（HPV）假病毒介导的基因转移来检测抗人乳头瘤病毒（HPV）的中和抗体。
J Clin Microbiol. 2002 Mar;40(3):926-32. doi: 10.1128/JCM.40.3.926-932.2002.
6
Nasal immunization of mice with human papillomavirus type 16 (HPV-16) virus-like particles or with the HPV-16 L1 gene elicits specific cytotoxic T lymphocytes in vaginal draining lymph nodes.用16型人乳头瘤病毒（HPV - 16）病毒样颗粒或HPV - 16 L1基因对小鼠进行鼻腔免疫，可在阴道引流淋巴结中引发特异性细胞毒性T淋巴细胞。
J Virol. 1999 Nov;73(11):9063-71. doi: 10.1128/JVI.73.11.9063-9071.1999.
本文引用的文献
1
The L1 major capsid protein of human papillomavirus type 16 variants affects yield of virus-like particles produced in an insect cell expression system.人乳头瘤病毒16型变体的L1主要衣壳蛋白影响昆虫细胞表达系统中产生的病毒样颗粒的产量。
J Clin Microbiol. 1998 Jul;36(7):2046-51. doi: 10.1128/JCM.36.7.2046-2051.1998.
2
Folding and assembly of hepatitis B virus core protein: a new model proposal.乙型肝炎病毒核心蛋白的折叠与组装：一种新的模型提议
J Struct Biol. 1997 Apr;118(3):189-96. doi: 10.1006/jsbi.1997.3846.
3
ELISA for antibody to hepatitis E virus (HEV) based on complete open-reading frame-2 protein expressed in insect cells: identification of HEV infection in primates.基于昆虫细胞中表达的戊型肝炎病毒（HEV）完整开放阅读框2蛋白的戊型肝炎病毒抗体酶联免疫吸附测定：灵长类动物中戊型肝炎病毒感染的鉴定
J Infect Dis. 1993 Aug;168(2):369-78. doi: 10.1093/infdis/168.2.369.
4
Three-dimensional structure of hepatitis B virus core particles determined by electron cryomicroscopy.通过电子冷冻显微镜确定的乙型肝炎病毒核心颗粒的三维结构。
Cell. 1994 Jun 17;77(6):943-50. doi: 10.1016/0092-8674(94)90142-2.
5
Mapping of linear B cell epitopes on open reading frames 2- and 3-encoded proteins of hepatitis E virus using synthetic peptides.利用合成肽对戊型肝炎病毒开放阅读框2和3编码蛋白上的线性B细胞表位进行定位
FEMS Microbiol Lett. 1993 May 15;109(2-3):251-5. doi: 10.1111/j.1574-6968.1993.tb06176.x.
6
Hybrid hepatitis B virus nucleocapsid bearing an immunodominant region from hepatitis B virus surface antigen.携带来自乙型肝炎病毒表面抗原免疫显性区域的杂交乙型肝炎病毒核衣壳。
J Virol. 1993 Jun;67(6):3696-701. doi: 10.1128/JVI.67.6.3696-3701.1993.
7
Insertions within the hepatitis B virus capsid protein influence capsid formation and RNA encapsidation.乙型肝炎病毒衣壳蛋白内的插入影响衣壳形成和RNA包装。
J Virol. 1995 Nov;69(11):6833-8. doi: 10.1128/JVI.69.11.6833-6838.1995.
8
Complete nucleotide sequence of hepatitis B virus.乙型肝炎病毒的完整核苷酸序列
Nucleic Acids Res. 1990 Aug 11;18(15):4587. doi: 10.1093/nar/18.15.4587.
9
Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.戊型肝炎病毒（HEV）：病毒全基因组的分子克隆与测序
Virology. 1991 Nov;185(1):120-31. doi: 10.1016/0042-6822(91)90760-9.
10
Hepatitis E virus: identification of type-common epitopes.戊型肝炎病毒：共同型表位的鉴定
J Virol. 1991 Nov;65(11):5790-7. doi: 10.1128/JVI.65.11.5790-5797.1991.
Zotero 插件