Manicardi G C, Tombacco A, Bizzaro D, Bianchi U, Bianchi P G, Sakkas D
Department of Animal Biology, University of Modena, Italy.
Histochem J. 1998 Jan;30(1):33-9. doi: 10.1023/a:1003214529185.
The nick translation and terminal transferase assays have been compared to test their relative efficiency in detecting DNA breakage in ejaculated human spermatozoa. The results have been correlated with the percentage of chromomycin A3 positive sperm, a fluorochrome that is indicative of the protamination state of sperm. Examination of the ejaculated sperm of 30 subjects revealed that the percentage of positivity to the nick translation and terminal transferase assays did not differ, even when using different fixatives. It is concluded that the inability of the two assays to distinguish the type of DNA damage, as is possible in somatic nuclei, is most probably linked to the unique nature of sperm chromatin. It is proposed that the presence of the damaged DNA may be the remnants of an imperfect spermiogenesis, probably related to an inadequate protamine deposition. This is supported by the strong correlation between the presence of DNA damage and underprotamination as evidenced by chromomycin A3.
已对缺口平移和末端转移酶检测法进行比较，以测试它们在检测射出的人类精子中DNA断裂方面的相对效率。结果已与嗜铬霉素A3阳性精子的百分比相关联，嗜铬霉素A3是一种指示精子鱼精蛋白状态的荧光染料。对30名受试者射出的精子进行检查发现，即使使用不同的固定剂，缺口平移和末端转移酶检测法的阳性百分比也没有差异。得出的结论是，这两种检测法无法像在体细胞核中那样区分DNA损伤类型，很可能与精子染色质的独特性质有关。有人提出，受损DNA的存在可能是精子发生不完善的残余物，可能与鱼精蛋白沉积不足有关。嗜铬霉素A3证明了DNA损伤的存在与鱼精蛋白不足之间的强相关性，这支持了上述观点。
