一种用于99mTc标记的葡聚糖试剂盒的研制及其淋巴闪烁显像评估

Development of a dextran kit for labelling with 99mTc and its evaluation for lymphoscintigraphy.

作者信息

Dass R S, Singh A K, Chauhan U P

机构信息

Department of Radiopharmaceuticals, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi, India.

出版信息

Nucl Med Biol. 1993 Jul;20(5):701-6. doi: 10.1016/0969-8051(93)90041-r.

DOI:10.1016/0969-8051(93)90041-r

PMID:7689385

Abstract

A cold dextran (molecular weight 60,000-90,000) kit has been developed by a modified procedure to produce instant preparation of 99mTc-dextran suitable for lymphoscintigraphy. The preparation was subjected to various quality control measures. Effect of pH and amount of stannous chloride as a reducing agent on the labelling efficiency using ITLC were studied. The labelled complex was separated from both the reduced pertechnetate and pertechnetate and quantified. In a series of experiments hydrolysed/reduced 99mTc was found to be less than 3.0%, whereas pertechnetate was approx. 1.0%. Biokinetics of the agent in mice and blood clearance and rate of disappearance from the site of intradermal injection of the agent in rabbits were studied. The tagged agent for imaging of the lymphatic system activated by administering Freund's complete adjuvant (FCA)/E. coli in rabbits exhibited its suitability for lymphoscintigraphy.

摘要

已通过改良程序开发出一种冷葡聚糖（分子量60,000 - 90,000）试剂盒，用于即时制备适用于淋巴闪烁显像的99mTc - 葡聚糖。该制剂经过了各种质量控制措施。研究了pH值和作为还原剂的氯化亚锡用量对使用ITLC的标记效率的影响。将标记复合物与还原高锝酸盐和高锝酸盐分离并进行定量。在一系列实验中，发现水解/还原的99mTc小于3.0%，而高锝酸盐约为1.0%。研究了该制剂在小鼠体内的生物动力学、血液清除情况以及在兔皮内注射部位的消失速率。在兔体内通过给予弗氏完全佐剂（FCA）/大肠杆菌激活的用于淋巴系统成像的标记制剂显示出其适用于淋巴闪烁显像。