Saboural Pierre, Chaubet Frédéric, Rouzet Francois, Al-Shoukr Faisal, Azzouna Rana Ben, Bouchemal Nadia, Picton Luc, Louedec Liliane, Maire Murielle, Rolland Lydia, Potier Guy, Guludec Dominique Le, Letourneur Didier, Chauvierre Cédric
Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France.
Laboratory CSPBAT, Paris 13 University, Sorbonne Paris Cité, CNRS UMR 7244, SBMB team, F-93017, Bobigny, France.
Mar Drugs. 2014 Sep 23;12(9):4851-67. doi: 10.3390/md12094851.
Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
岩藻聚糖是一类具有多种生化特性的硫酸化多糖大家族。一种来自褐藻的商业岩藻聚糖，含有由L-岩藻糖、糖醛酸和硫酸基团组成的低分子量多糖类物质，在此仅用醋酸钙溶液进行处理。该处理得到了一个纯化级分，产率为45%。使用比色法、多角度激光光散射（MALLS）、示差折光检测（dRI）、傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）对纯化后的岩藻聚糖进行的物理化学表征显示，两种岩藻聚糖的分子量分布和化学谱相似，而硫酸根和L-岩藻糖含量分别增加了16%和71%。对用99mTc标记的两种化合物在大鼠体内进行的生物分布研究表明，纯化后的岩藻聚糖主要经肾脏排泄，而粗岩藻聚糖主要滞留在肝脏和脾脏中。在大鼠心肌缺血再灌注实验中，我们随后证明了纯化后的岩藻聚糖具有更好的效果。这种纯化后的硫酸化多糖在急性冠状动脉综合征分子成像的开发方面似乎很有前景。
