Olovsson M, Nilsson B O
Department of Human Anatomy, Biomedical Center, Uppsala University, Sweden.
Hybridoma. 1993 Jun;12(3):305-15. doi: 10.1089/hyb.1993.12.305.
Monoclonal antibodies recognizing epitopes expressed by invasive mouse trophoblast cells were obtained by immunizing male mice of the NMRI strain with mouse egg-cylinders obtained by culturing blastocysts on agarose for 4 days. Immunization was achieved by intrasplenic and in vitro methods. The procedures described outline a rational way of producing and selecting antibodies which are stage-specific for the mouse trophoblast at implantation and early placentation. Preimplantation stage-specificity was tested and adhesive blastocysts only were detected by 4 antibodies. Thus, these antibodies become markers for that developmental stage which characterizes an adhesive blastocyst ready to implant. Postimplantation stage-specificity was demonstrated by 6 antibodies, which labelled trophoblast cells of day 7 implantations only. Thus, there is a specific expression of some epitopes by the invasive trophoblast. Cell surface epitopes were detected on adhesive blastocysts by 4 antibodies and on the invasive trophoblast of the egg-cylinder by 7 antibodies. Shedded antigens from egg-cylinders in vitro were detected by 5 antibodies. Four of these antibodies detected substances released only from early implantation stages. A functional effect was demonstrated for an IgM antibody which impaired hatching and outgrowth of blastocysts in vitro.
通过用在琼脂糖上培养4天的囊胚所获得的小鼠卵柱免疫NMRI品系的雄性小鼠，获得了识别侵袭性小鼠滋养层细胞表达的表位的单克隆抗体。免疫是通过脾内和体外方法实现的。所描述的程序概述了一种生产和选择对植入和早期胎盘形成时的小鼠滋养层具有阶段特异性的抗体的合理方法。测试了植入前阶段特异性，仅4种抗体检测到附着的囊胚。因此，这些抗体成为表征准备植入的附着囊胚的那个发育阶段的标志物。6种抗体证明了植入后阶段特异性，这些抗体仅标记第7天植入的滋养层细胞。因此，侵袭性滋养层存在一些表位的特异性表达。4种抗体在附着囊胚上检测到细胞表面表位，7种抗体在卵柱的侵袭性滋养层上检测到细胞表面表位。5种抗体检测到体外卵柱脱落的抗原。其中4种抗体检测到仅从早期植入阶段释放的物质。证明了一种IgM抗体在体外损害囊胚孵化和生长的功能作用。
