Nilsson B O, Svalander P C, Andersson J, Grönvik K O, Larsson A
Ups J Med Sci. 1986;91(3):317-22. doi: 10.3109/03009738609178672.
Monoclonal antibodies against mouse blastocysts in the adhesion stage of implantation were obtained by intrasplenic immunization with blastocysts either injected into the spleen after irradiation or transferred after drying on a piece of nitrocellulose paper. About 10 blastocysts were deposited at each of 4 immunizations before the spleen cells were used for hybridoma production. The supernatants obtained were examined for anti-blastocyst antibodies in indirect immunofluorescence labelling of native blastocysts and with ABC-immunoperoxidase staining either of blastocysts attached to nitrocellulose paper or of methanol-fixed blastocysts. Further, sections of paraffin-embedded blastocysts were used for detection of antigens.
通过脾内免疫法获得针对植入黏附阶段小鼠囊胚的单克隆抗体，囊胚通过以下两种方式进行免疫：一是经辐照后注入脾脏，二是干燥于硝酸纤维素纸上后转移至脾脏。在将脾细胞用于制备杂交瘤之前，每次免疫接种约10个囊胚，共进行4次免疫接种。所得上清液通过对天然囊胚进行间接免疫荧光标记以及对附着于硝酸纤维素纸上的囊胚或甲醇固定的囊胚进行ABC免疫过氧化物酶染色来检测抗囊胚抗体。此外，石蜡包埋囊胚的切片用于抗原检测。
