Hancock R E, Wieczorek A A, Mutharia L M, Poole K
Infect Immun. 1982 Jul;37(1):166-71. doi: 10.1128/iai.37.1.166-171.1982.
Hybridomas secreting monoclonal antibodies specific for Pseudomonas aeruginosa outer membrane antigens were isolated. One of the antibodies was highly specific for the O antigen of the lipopolysaccharide of International Antigen Typing Scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotypes. This monoclonal antibody was able to agglutinate heat-killed bacterial cells as well as lipopolysaccharide-coated sheep erythrocytes. Two other monoclonal antibodies were able to interact with the outer membranes of strains representing all 17 serotypes, although they were unable to agglutinate heat-killed bacterial cells. One of these was shown to be specific for the major outer membrane lipoprotein H2. The antigenic site against which this monoclonal antibody reacted was present in the outer membranes of two Pseudomonas fluorescens strains, two Pseudomonas putida strains, a Pseudomonas anguilliseptica strain, and an Azotobacter vinelandii strain, but not in the outer membranes of five other bacterial species.
分离出了分泌对铜绿假单胞菌外膜抗原有特异性的单克隆抗体的杂交瘤。其中一种抗体对国际抗原分型方案血清型5菌株脂多糖的O抗原有高度特异性，与血清型17菌株反应微弱，且不与代表其他15种血清型的菌株外膜发生反应。这种单克隆抗体能够凝集热杀死的细菌细胞以及脂多糖包被的绵羊红细胞。另外两种单克隆抗体能够与代表所有17种血清型的菌株外膜相互作用，尽管它们不能凝集热杀死的细菌细胞。其中一种被证明对主要外膜脂蛋白H2具有特异性。该单克隆抗体所反应的抗原位点存在于两株荧光假单胞菌、两株恶臭假单胞菌、一株鳗弧假单胞菌和一株棕色固氮菌的外膜中，但不存在于其他五种细菌的外膜中。
