Péault B, Weissman I, Baum C
Systemix Inc., Palo Alto, CA 94303.
Leukemia. 1993 Aug;7 Suppl 2:S98-101.
Immune deficient SCID mice support the hematopoietic development of surgically implanted human fetal thymus and bone. The usability of the resulting SCID-hu hematolymphoid chimaera as an assay system for human candidate blood stem cells was suggested by the observation that allogeneic CD34+ cells sorted from fetal liver and bone marrow can engraft and differentiate in the transplanted human thymus and bone marrow. The SCID-hu mouse has been further used, in combination with a mouse bone marrow stromal cell line that supports human myeloid and B lymphoid differentiation in vitro, to identify a minor subset of fetal CD34-expressing cells that exhibit multilineage hematopoietic potentialities.
免疫缺陷的重症联合免疫缺陷（SCID）小鼠能够支持手术植入的人类胎儿胸腺和骨髓的造血发育。从胎儿肝脏和骨髓中分选出来的同种异体CD34+细胞能够在移植的人类胸腺和骨髓中植入并分化，这一观察结果表明，由此产生的SCID-hu血液淋巴嵌合体可作为人类候选血液干细胞的检测系统。SCID-hu小鼠还与一种在体外支持人类髓系和B淋巴细胞分化的小鼠骨髓基质细胞系联合使用，以鉴定一小部分表达胎儿CD34的细胞亚群，这些细胞具有多谱系造血潜能。
