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Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.

作者信息

Mark D F, Richardson C C

出版信息

Proc Natl Acad Sci U S A. 1976 Mar;73(3):780-4. doi: 10.1073/pnas.73.3.780.

DOI:10.1073/pnas.73.3.780
PMID:768986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336002/
Abstract

T7 DNA polymerase (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) is composed of an 84,000 dalton protein specified by the gene 5 of the phage and a 12,000 dalton protein (TsnC protein) specified by the tsnC gene of E. coli [Modrich, P. & Richardson, C. C. (1975) J. Biol. Chem. 250 5515-5522]. Both proteins are necessary for T7 DNA polymerase activity and for the replication of T7 DNA. The TsnC protein is identical to thioredoxin of E. coli by the following criteria: (1) Homogeneous preparations of both proteins have TsnC and thioredoxin activity. (2) Both proteins show similar stability to heat. (3) They have identical mobilities, corresponding to a molecular weight of 12,000, on polyacrylamide gels containing sodium dodecyl sulfate. (4) Their amino-acid compositions are indistinguishabe. (5) Antibody prepared against thioredoxin inhibits TsnC activity. (6) TsnC protein isolated from purified T7 DNA polymerase has thioredoxin activity. In addition, preparations of T7 DNA polymerase itself exhibit thioredoxin activity and are partially inhibited by antibody to thioredoxin.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace1/336002/75c665d0b45c/pnas00672-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace1/336002/75c665d0b45c/pnas00672-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace1/336002/75c665d0b45c/pnas00672-0123-a.jpg

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Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.
Proc Natl Acad Sci U S A. 1976 Mar;73(3):780-4. doi: 10.1073/pnas.73.3.780.
2
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本文引用的文献

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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
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Insights into the structural dynamics of the bacteriophage T7 DNA polymerase and its complexes.对噬菌体T7 DNA聚合酶及其复合物结构动力学的见解。
J Mol Model. 2018 Jun 1;24(7):144. doi: 10.1007/s00894-018-3671-2.
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The thioredoxin system and not the Michaelis-Menten equation should be fitted to substrate saturation datasets from the thioredoxin insulin assay.硫氧还蛋白系统而非米氏方程应适用于硫氧还蛋白胰岛素测定的底物饱和数据集。
Redox Rep. 2016 Jul;21(4):170-9. doi: 10.1179/1351000215Y.0000000024. Epub 2016 Mar 21.
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Diverse virulent pneumophages infect Streptococcus mitis.多种烈性肺炎噬菌体感染缓症链球菌。
PLoS One. 2015 Feb 18;10(2):e0118807. doi: 10.1371/journal.pone.0118807. eCollection 2015.
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J Virol. 1974 Sep;14(3):509-16. doi: 10.1128/JVI.14.3.509-516.1974.