无法支持T7在细胞内生长的大肠杆菌原养型突变体的分离与鉴定
Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7.
作者信息
Chamberlin M
出版信息
J Virol. 1974 Sep;14(3):509-16. doi: 10.1128/JVI.14.3.509-516.1974.
Abstract
Mutants of E. coli B/1 were isolated which grew normally but did not permit the intracellular growth of bacteriophage T7. Two classes of mutants were studied in detail (tsnB(-) and tsnC(-)). These strains adsorbed T7 normally and were killed by the infection. Synthesis of T7 RNA and of early and late classes of T7 proteins occurred normally after infection. In T7-infected tsnB(-) cells, T7 DNA synthesis stopped prematurely shortly after its onset, suggesting that the tsnB function affects a step in the late phase of T7 DNA replication. Mutants of T7 were isolated (T7beta) which could grow on tsnB(-) cells. In T7-infected tsnC(-) cells, T7 DNA synthesis was completely blocked, suggesting that the tsnC function affects a step in an early phase of T7 DNA replication.
摘要
分离出了大肠杆菌B/1的突变体，它们生长正常，但不允许噬菌体T7在细胞内生长。详细研究了两类突变体（tsnB(-)和tsnC(-)）。这些菌株能正常吸附T7，并在感染后被杀死。感染后，T7 RNA以及早期和晚期T7蛋白的合成正常发生。在被T7感染的tsnB(-)细胞中，T7 DNA合成在开始后不久就过早停止，这表明tsnB功能影响T7 DNA复制后期的一个步骤。分离出了能在tsnB(-)细胞上生长的T7突变体（T7beta）。在被T7感染的tsnC(-)细胞中，T7 DNA合成被完全阻断，这表明tsnC功能影响T7 DNA复制早期的一个步骤。
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