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长末端重复序列及侧翼细胞DNA中的序列对逆转录病毒转录本聚腺苷酸化的影响。

Influence of sequences in the long terminal repeat and flanking cell DNA on polyadenylation of retroviral transcripts.

作者信息

Swain A, Coffin J M

机构信息

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.

出版信息

J Virol. 1993 Oct;67(10):6265-9. doi: 10.1128/JVI.67.10.6265-6269.1993.

DOI:10.1128/JVI.67.10.6265-6269.1993
PMID:7690423
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238050/
Abstract

Readthrough transcripts are formed during retrovirus infection by polyadenylation of viral RNA in cellular sequences adjacent to the provirus. We have studied such transcripts in avian leukosis virus-infected cell clones containing a single provirus, either the wild type or one with an inactivating mutation in the poly(A) addition signal. All individual wild-type proviruses produced readthrough transcripts, implying that this property is not restricted to a few integration sites. The range of sizes of viral RNA in the mutant lacking a correct signal for poly(A) addition reflected both the occurrence of functional polyadenylation sites within flanking cell DNA and increased usage of cryptic sites within viral sequences.

摘要

通读转录本是在逆转录病毒感染过程中，通过病毒RNA在原病毒相邻细胞序列中的多聚腺苷酸化形成的。我们研究了禽白血病病毒感染的细胞克隆中的此类转录本，这些克隆含有单个原病毒，要么是野生型，要么是在聚腺苷酸添加信号中具有失活突变的原病毒。所有单个野生型原病毒都产生通读转录本，这意味着该特性并不局限于少数整合位点。缺乏正确聚腺苷酸添加信号的突变体中病毒RNA的大小范围既反映了侧翼细胞DNA内功能性聚腺苷酸化位点的出现，也反映了病毒序列内隐蔽位点使用的增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/2665a1bb958a/jvirol00031-0570-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/e8833135bf19/jvirol00031-0568-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/fd1128bf5606/jvirol00031-0569-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/2665a1bb958a/jvirol00031-0570-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/e8833135bf19/jvirol00031-0568-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/fd1128bf5606/jvirol00031-0569-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b4/238050/2665a1bb958a/jvirol00031-0570-a.jpg

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本文引用的文献

1
Site-specific mutagenesis of avian erythroblastosis virus: erb-B is required for oncogenicity.禽成红细胞增多症病毒的位点特异性诱变：致癌性需要erb-B。
Virology. 1983 Oct 15;130(1):155-78. doi: 10.1016/0042-6822(83)90125-3.
2
Differential transcription from the long terminal repeats of integrated avian leukosis virus DNA.整合禽白血病病毒DNA长末端重复序列的差异转录
J Virol. 1986 Nov;60(2):497-505. doi: 10.1128/JVI.60.2.497-505.1986.
3
Transcription termination and 3' processing: the end is in site!转录终止与3' 加工：终点就在眼前！
J Virol. 1997 Mar;71(3):2346-56. doi: 10.1128/JVI.71.3.2346-2356.1997.
4
Functional and biological properties of an avian variant long terminal repeat containing multiple A to G conversions in the U3 sequence.一种在U3序列中含有多个A到G转换的禽类变异长末端重复序列的功能和生物学特性。
J Virol. 1994 Aug;68(8):4759-67. doi: 10.1128/JVI.68.8.4759-4767.1994.
5
Retroviral sequences located within an intron of the dilute gene alter dilute expression in a tissue-specific manner.位于稀释基因内含子内的逆转录病毒序列以组织特异性方式改变稀释表达。
EMBO J. 1995 May 15;14(10):2326-32. doi: 10.1002/j.1460-2075.1995.tb07227.x.
6
A nonproducer, interfering human immunodeficiency virus (HIV) type 1 provirus can be transduced through a murine leukemia virus-based retroviral vector: recovery of an anti-HIV mouse/human pseudotype retrovirus.一种无病毒产生能力、具有干扰性的人类免疫缺陷病毒1型前病毒可通过基于鼠白血病病毒的逆转录病毒载体进行转导：抗HIV小鼠/人类假型逆转录病毒的恢复。
J Virol. 1995 Nov;69(11):6618-26. doi: 10.1128/JVI.69.11.6618-6626.1995.
Cell. 1985 Jun;41(2):349-59. doi: 10.1016/s0092-8674(85)80007-6.
4
Polyadenylation at correct sites in genome RNA is not required for retrovirus replication or genome encapsidation.逆转录病毒复制或基因组衣壳化并不需要基因组RNA在正确位点进行多聚腺苷酸化。
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5
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6
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7
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