Picerno G, Fiocca S
Quad Sclavo Diagn. 1975 Sep;11(3):661-8.
In the present work, 21,400 not selected sera were tested with hemagglutination test (TPHA) and flocculation test (VDRL) with cardiolipin for screening of syphilis; sera that gave negative results against both tests showed remarkable agreement (97,81%). 468 sera gave positive results to least one of the tests employed in screening and were tested with the following procedures: fluorescent Treponemal antibody-absorption (FTA/ABS) test, complement fixation (by Kolmer, modified) with proteic (KP) and lipidic (KL) antigen; 100 of these sera were also tested with T. pallidum immobilizaiton (TPI) test. The agreement among the before mentioned tests and sera that gave positive results only to VDRL was significatively low; on the contrary, the agreement was remarkable in the case of those positive only to TPHA (99% with FTA/ABS, 83% with TPI, 52,5% with KP and 21,5% with KL). The results obtained in attesting sensitivity and specificity of TPHA indicate that it is a very valuable screening test, and suggest to the AA. to emphasize that TPHA/VDRL association a definitive conclusion in syphilis serological negativity.
在本研究中，对21400份未经筛选的血清进行了梅毒螺旋体血凝试验（TPHA）和用卵磷脂进行的絮状试验（VDRL）以筛查梅毒；两种试验结果均为阴性的血清显示出高度一致性（97.81%）。468份血清在筛查中至少有一种试验呈阳性，并采用以下方法进行检测：荧光螺旋体抗体吸收（FTA/ABS）试验、用蛋白抗原（KP）和脂质抗原（KL）进行的补体结合试验（改良柯尔默试验）；其中100份血清还进行了梅毒螺旋体制动（TPI）试验。上述试验与仅VDRL呈阳性的血清之间的一致性显著较低；相反，仅TPHA呈阳性的血清一致性显著（与FTA/ABS为99%，与TPI为83%，与KP为52.5%，与KL为21.5%）。证实TPHA敏感性和特异性所获得的结果表明它是一种非常有价值的筛查试验，并建议作者强调TPHA/VDRL联合检测对梅毒血清学阴性得出明确结论。
