Zanelli E, Henry M, Malthièry Y
Laboratorie de Biochimie Médicale, INSERM U38, Faculté de Médecine, Marseille, France.
Cell Mol Biol (Noisy-le-grand). 1993 Jul;39(5):491-501.
Human thyroid peroxidase (hTPO) is the major component of the microsomal antigen. In almost cases, antibodies against this protein are found in sera from patients with autoimmune thyroid diseases. Overlapping cDNAs which correspond to the complete hTPO mRNA obtained from a thyroid library or by polymerase chain reaction were cloned and expressed as fusion proteins in a prokaryotic vector. Seven antigenic determinants between 21 and 49 amino acids were defined by cloning, subcloning of the immunoreactive regions and screenings with the two rabbit polyclonal anti hTPO antibodies. This study confirms the antigenic nature of the sequences 70-160 and 590-675 but above all refines the localization of three shorter distinct antigenic peptides corresponding to the sequences 68-105, 106-126 and 574-621. Moreover, four other determinants were characterized on the sequences 233-277, 467-515, 641-685 and 701-730. Analysis of sera from patients with autoimmune thyroid diseases (AITD) against the seven immunoreactive peptides confirms the heterogeneous nature of autoantibodies to hTPO.
人甲状腺过氧化物酶（hTPO）是微粒体抗原的主要成分。在几乎所有情况下，自身免疫性甲状腺疾病患者的血清中都能检测到针对该蛋白的抗体。从甲状腺文库或通过聚合酶链反应获得的对应于完整hTPO mRNA的重叠cDNA被克隆，并在原核载体中作为融合蛋白表达。通过对免疫反应区域进行克隆、亚克隆以及用两种兔多克隆抗hTPO抗体进行筛选，确定了7个21至49个氨基酸之间的抗原决定簇。本研究证实了70 - 160和590 - 675序列的抗原性质，但最重要的是细化了对应于68 - 105、106 - 126和574 - 621序列的三个较短的不同抗原肽的定位。此外，还在233 - 277、467 - 515、641 - 685和701 - 730序列上鉴定了另外四个决定簇。对自身免疫性甲状腺疾病（AITD）患者血清针对这7种免疫反应性肽的分析证实了抗hTPO自身抗体的异质性。
