自身免疫性甲状腺疾病患者血清中抗钠/碘同向转运体自身抗体。

Autoantibody against Na+/I- symporter in the sera of patients with autoimmune thyroid disease.

作者信息

Endo T, Kogai T, Nakazato M, Saito T, Kaneshige M, Onaya T

机构信息

Third Department of Internal Medicine, Yamanashi Medical University, Japan.

出版信息

Biochem Biophys Res Commun. 1996 Jul 5;224(1):92-5. doi: 10.1006/bbrc.1996.0989.

DOI:10.1006/bbrc.1996.0989

PMID:8694839

Abstract

Using recombinant rat Na+/I- symporter (NaIS) protein, we have immunochemically searched for the autoantibody in the sera from patients with autoimmune thyroid disease. We found that 22 out of 26 sera (84%) from patients with Graves' disease and 3 out of 20 sera (15%) from patients with Hashimoto's thyroiditis recognized it. By Western blot, these IgGs reacted with 80 kDa protein in FRTL-5 cell membrane, which co-migrated with the band stained by rabbit antibody toward NaIS. These results indicate that autoantibody against NaIS, newly identified antibody, frequently exists in patients with autoimmune thyroid disease, especially in Graves' disease.

摘要

利用重组大鼠钠/碘同向转运体（NaIS）蛋白，我们通过免疫化学方法在自身免疫性甲状腺疾病患者的血清中寻找自身抗体。我们发现，26例格雷夫斯病患者血清中有22例（84%）、20例桥本甲状腺炎患者血清中有3例（15%）能识别该蛋白。通过蛋白质印迹法，这些免疫球蛋白与FRTL-5细胞膜中的80 kDa蛋白发生反应，该蛋白与兔抗NaIS抗体染色的条带迁移情况一致。这些结果表明，新鉴定出的针对NaIS的自身抗体在自身免疫性甲状腺疾病患者中经常存在，尤其是在格雷夫斯病患者中。