Yang G C, Brooks J J, Roberts S, Gupta P K
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia.
Cancer. 1993 Oct 1;72(7):2260-5. doi: 10.1002/1097-0142(19931001)72:7<2260::aid-cncr2820720733>3.0.co;2-9.
Acquired immunodeficiency syndrome (AIDS)-associated Kaposi sarcoma (KS) cells have not been reported in pleural effusions. This study identifies the effusional form of AIDS-KS cells with the CD34 antibody, a newly recognized marker for vascular neoplasia.
In the pleural effusion of a patient with AIDS and biopsy proven pulmonary KS, the authors found bizarre amoeboid cells. Parallel sections from the cell blocks of the pleural effusions from the index patient and six other patients with AIDS were immunostained for cytokeratin, CD68, leukocyte common antigen (LCA), Factor VII:R, and CD34.
The atypical cells were not observed in the pleural effusions of the other six patients with AIDS. The atypical cells were positive for CD34 (4+) and Factor VIII:R (1+) but were negative for cytokeratin, CD68, or LCA, which were expressed by mesothelial cells, macrophages, and lymphocytes, respectively. The expression for CD34 and Factor VIII:R was limited to a sharply delineated perinuclear region in the cytoplasm, corresponding to the erythrocyte-containing intracytoplasmic space of the atypical cells on the filter.
In conclusion, the erythrocyte-containing intracytoplasmic space within the atypical cells most likely represents the intracytoplasmic lumina of the AIDS-KS endothelial cells and the CD-34-positive atypical cells represent the effusional form of the AIDS-KS cells.
据报道，在胸腔积液中尚未发现获得性免疫缺陷综合征（AIDS）相关的卡波西肉瘤（KS）细胞。本研究使用CD34抗体鉴定了AIDS-KS细胞的积液形式，CD34是一种新发现的血管肿瘤标志物。
在一名经活检证实患有肺部KS的AIDS患者的胸腔积液中，作者发现了奇异的阿米巴样细胞。对该索引患者以及其他6名AIDS患者胸腔积液细胞块的平行切片进行细胞角蛋白、CD68、白细胞共同抗原（LCA）、因子VII：R和CD34免疫染色。
在其他6名AIDS患者的胸腔积液中未观察到非典型细胞。非典型细胞CD34呈阳性（4+），因子VIII：R呈阳性（1+），但细胞角蛋白、CD68或LCA呈阴性，而间皮细胞、巨噬细胞和淋巴细胞分别表达这些物质。CD34和因子VIII：R的表达仅限于细胞质中界限分明的核周区域，与滤膜上非典型细胞含红细胞的胞质空间相对应。
总之，非典型细胞内含有红细胞的胞质空间很可能代表AIDS-KS内皮细胞的胞质内腔，而CD-34阳性非典型细胞代表AIDS-KS细胞的积液形式。
