Moretti G, Betto P, Cammarata P, Fracassi F, Giambenedetti M, Borghese A
Istituto Superiore di Sanita, Rome, Italy.
J Chromatogr. 1993 Jul 2;616(2):291-6. doi: 10.1016/0378-4347(93)80397-m.
A procedure is described for the concurrent assay of thiouracil, methylthiouracil, propylthiouracil, phenylthiouracil and methimazole in bovine plasma. In this procedure, reversed-phase high-performance liquid chromatography is performed after liquid-liquid extraction of plasma with ethyl acetate. Compounds are quantified by ultraviolet detection using a wavelength of 276 nm for thiouracil, methylthiouracil, propylthiouracil and phenylthiouracil and 258 nm for methimazole. The linearity range, precision, recovery and detection limits were determined and the method was shown to be applicable to samples of plasma from young bulls experimentally treated with methylthiouracil.
本文描述了一种同时测定牛血浆中硫脲嘧啶、甲硫氧嘧啶、丙硫氧嘧啶、苯硫氧嘧啶和甲巯咪唑的方法。在此方法中，用乙酸乙酯对血浆进行液-液萃取后，进行反相高效液相色谱分析。通过紫外检测对化合物进行定量，硫脲嘧啶、甲硫氧嘧啶、丙硫氧嘧啶和苯硫氧嘧啶使用276 nm波长，甲巯咪唑使用258 nm波长。测定了线性范围、精密度、回收率和检测限，结果表明该方法适用于经甲硫氧嘧啶实验处理的青年公牛的血浆样本。
