Buick R K, Barry C, Traynor I M, McCaughey W J, Elliott C T
Department of Agriculture for Northern Ireland, Veterinary Science Division, Belfast, UK.
J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):71-9. doi: 10.1016/s0378-4347(98)00453-8.
High-performance liquid chromatography (HPLC) methodologies were evaluated for the detection and quantification of thyreostatic drug residues in cattle serum and thyroid tissue. The paper details a protocol, using a simple ethyl acetate extraction for the determination of thiouracil, tapazole, methyl thiouracil, propyl thiouracil and phenyl thiouracil in thyroid tissue. Using two sequential HPLC injections, and quantitative analysis, in two steps, all five thyreostats were detectable at concentrations greater than 2.45-4.52 ng/g. Modifications to a published method for detection of thyreostatic residues in serum involving the addition of mercaptoethanol and a freezing step are described. The modifications improved sensitivity and allowed detection of the five thyreostats at levels greater than 16.98-35.25 ng/ml. Young bulls were treated with thyreostats to demonstrate the validity of the methodologies described. Administered thyreostats were not absorbed equally by the test animals and the compounds were not all detected in the serum samples removed at 7 days following drug withdrawal. These experiments indicate the necessity to be able to detect thyreostat residues in a variety of matrices.
对高效液相色谱（HPLC）方法进行了评估，以检测和定量牛血清及甲状腺组织中的抗甲状腺药物残留。本文详细介绍了一种方案，该方案采用简单的乙酸乙酯萃取法测定甲状腺组织中的硫脲嘧啶、甲巯咪唑、甲基硫脲嘧啶、丙基硫脲嘧啶和苯基硫脲嘧啶。通过两次连续的HPLC进样以及两步定量分析，所有五种抗甲状腺药物在浓度大于2.45 - 4.52 ng/g时均可检测到。文中还描述了对已发表的血清中抗甲状腺药物残留检测方法的改进，包括添加巯基乙醇和冷冻步骤。这些改进提高了灵敏度，使五种抗甲状腺药物在浓度大于16.98 - 35.25 ng/ml时能够被检测到。用抗甲状腺药物处理幼公牛，以证明所描述方法的有效性。受试动物对所施用的抗甲状腺药物吸收并不相同，并且在停药7天后采集的血清样本中并非所有化合物都能被检测到。这些实验表明能够在多种基质中检测抗甲状腺药物残留的必要性。
