Willoughby J J, Ninkina N N, Beech M M, Latchman D S, Wood J N
Sandoz Institute for Medical Research, London, UK.
Neurosci Lett. 1993 Jun 11;155(2):136-9. doi: 10.1016/0304-3940(93)90691-d.
Three cDNA clones homologous to rat neuronal nicotinic receptors were identified in a human brain stem library by screening at low stringency with a mixture of rat alpha 5, beta 2 and beta 4 subunit probes. Positive clones were further analysed by hybridisation at higher stringency with individual rat nicotinic receptor probes. One positive clone was found to encode a protein that exhibited characteristic features of a member of the ligand-gated ion channel class of protein subunits, and showed 89% homology with a rat beta 3 neuronal nicotinic receptor subunit. Northern blots demonstrated the presence of a 1.7 kb transcript in RNA extracted from adult human Pons. This clone has therefore been designated the human beta 3 neuronal nicotinic receptor subunit.
通过用大鼠α5、β2和β4亚基探针混合物进行低严谨度筛选，在人脑干文库中鉴定出了三个与大鼠神经元烟碱受体同源的cDNA克隆。通过用单个大鼠烟碱受体探针进行更高严谨度的杂交，对阳性克隆进行了进一步分析。发现一个阳性克隆编码一种蛋白质，该蛋白质表现出配体门控离子通道类蛋白质亚基成员的特征，并且与大鼠β3神经元烟碱受体亚基具有89%的同源性。Northern印迹显示从成人脑桥提取的RNA中存在1.7 kb的转录本。因此，该克隆被命名为人β3神经元烟碱受体亚基。
