Fornasari D, Chini B, Tarroni P, Clementi F
Department of Medical Pharmacology, University of Milan, Italy.
Neurosci Lett. 1990 Apr 6;111(3):351-6. doi: 10.1016/0304-3940(90)90287-j.
Neuronal nicotinic receptors (nAchRs) have been isolated or cloned in insect, bird and mammalian neurons, but no information exists on the primary structure of human neuronal nAchRs. By screening a cDNA library from the human neuroblastoma cell line IMR 32 with a cDNA probe corresponding to the full length of rat alpha 3-nicotinic subunit, we have identified an open reading frame encoding a protein of 502 amino acids. This protein shows all the features of members of the ligand-gated receptor superfamily and has two cysteine residues at positions 192, 193 which are typical of the nicotinic alpha-subunits. Because of its high homology to rat alpha 3 (93% amino acid identity), we conclude that we have cloned the human alpha 3-nicotinic subunit.
神经元烟碱型受体（nAchRs）已在昆虫、鸟类和哺乳动物神经元中被分离或克隆，但关于人类神经元nAchRs一级结构的信息尚不存在。通过用对应大鼠α3-烟碱型亚基全长的cDNA探针筛选人神经母细胞瘤细胞系IMR 32的cDNA文库，我们鉴定出一个编码502个氨基酸蛋白质的开放阅读框。该蛋白质具有配体门控受体超家族成员的所有特征，并且在192、193位有两个半胱氨酸残基，这是烟碱型α亚基的典型特征。由于其与大鼠α3具有高度同源性（93%氨基酸同一性），我们得出结论，我们已克隆出人类α3-烟碱型亚基。
