Christensen M E, Therkildsen M H, Poulsen S S, Bretlau P
Department of Otolaryngology, Rigshospitalet, University of Copenhagen, Denmark.
Acta Otolaryngol. 1993 Jul;113(4):563-7. doi: 10.3109/00016489309135864.
Fifteen laryngeal squamous cell carcinomas were investigated for the presence of transforming growth factor alpha (TGF-alpha) and epidermal growth factor (EGF) using immunohistochemical methods. In a recent study the same material was characterized for epidermal growth factor receptors (EGF receptors) which were confined predominantly to the undifferentiated cells. The expression of this growth factor system in malignant cells may play a role in carcinogenesis and/or tumour growth. All carcinomas were positive for TGF-alpha and 12 were positive for EGF. In moderately-to-well differentiated carcinomas, the immunoreactivity was mainly detected in the cytologically more differentiated cells. Nine sections included both laryngeal stratified squamous epithelium of normal appearance and carcinoma. The immunoreactivity was here again localized in the cytologically more differentiated cells above the basal cell layer. The present investigation and our previous results confirm the existence of EGF receptors, TGF-alpha and EGF in laryngeal carcinomas. In addition, we conclude that the conditions do exist for growth factors to act through an autocrine system in poorly differentiated tumours and through a paracrine system in the moderately-to-well differentiated tumours.
采用免疫组化方法对15例喉鳞状细胞癌进行转化生长因子α（TGF-α）和表皮生长因子（EGF）检测。在最近一项研究中，对相同材料进行了表皮生长因子受体（EGF受体）检测，结果显示其主要局限于未分化细胞。该生长因子系统在恶性细胞中的表达可能在致癌过程和/或肿瘤生长中发挥作用。所有癌组织TGF-α均呈阳性，12例EGF呈阳性。在中分化至高分化癌中，免疫反应主要在细胞学上分化较好的细胞中检测到。9个切片同时包含外观正常的喉复层鳞状上皮和癌组织。免疫反应再次定位于基底细胞层上方细胞学上分化较好的细胞中。本研究及我们之前的结果证实了喉癌中存在EGF受体、TGF-α和EGF。此外，我们得出结论，生长因子确实有可能通过自分泌系统在低分化肿瘤中发挥作用，并通过旁分泌系统在中分化至高分化肿瘤中发挥作用。
