Christensen M E, Therkildsen M H, Hansen B L, Hansen G N, Bretlau P
Department of Oto-Laryngology and Head & Neck Surgery, Rigshospitalet, University of Copenhagen, Denmark.
Acta Otolaryngol. 1992;112(4):734-8. doi: 10.3109/00016489209137467.
Laryngeal squamous cell carcinomas from 15 consecutive preoperatively irradiated patients were investigated for the expression of epidermal growth factor receptor (EGF receptor). The study was performed on frozen sections by means of the 5-layer APAAP technique employing an antibody recognizing the extracellular part of the EGF receptor. In sections from 9 of the patients with laryngeal squamous cell carcinoma, normal differentiated epithelia were included. Sections from 6 of these patients, in addition, contained dysplastic epithelia. Expression of EGF receptor-like material was demonstrated in the basal cell layer of normally differentiated laryngeal epithelial and in dysplastic epithelia. Fourteen of the squamous cell carcinomas proved EGF receptor positive. Nearly all cells in the poorly differentiated carcinomas showed positive staining with the antibodies. In moderately to well differentiated carcinomas a reduction in the extent of staining was seen in certain areas. Especially for the epithelial pearls, the staining reaction was localized to the undifferentiated cells in the periphery. This finding corresponds to the staining pattern observed in the basal cell layers of normal epithelial. The present investigation confirms the expression of EGF receptor-like material in normal laryngeal epithelial, dysplastic epithelial and squamous cell carcinoma. The staining pattern was similar to that observed in oral squamous cell carcinomas, predominantly varying inversely with cellular differentiation.
对15例术前接受过放疗的连续性喉鳞状细胞癌患者的样本进行研究，以检测表皮生长因子受体（EGF受体）的表达情况。研究采用五层碱性磷酸酶抗碱性磷酸酶（APAAP）技术，在冰冻切片上进行，所用抗体可识别EGF受体的细胞外部分。在15例喉鳞状细胞癌患者中，9例患者的切片包含正常分化的上皮组织。此外，其中6例患者的切片含有发育异常的上皮组织。在正常分化的喉上皮基底细胞层和发育异常的上皮组织中均检测到EGF受体样物质的表达。14例鳞状细胞癌样本显示EGF受体呈阳性。在低分化癌中，几乎所有细胞均对抗体呈阳性染色。在中分化至高分化癌中，某些区域的染色范围有所减少。特别是对于上皮珠，染色反应定位于周边未分化细胞。这一发现与正常上皮基底细胞层中观察到的染色模式一致。本研究证实了EGF受体样物质在正常喉上皮、发育异常上皮和鳞状细胞癌中的表达。其染色模式与口腔鳞状细胞癌中观察到的相似，主要与细胞分化程度呈负相关。
